EasySep® Human Whole Blood CD45 Depletion Kit

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Immunomagnetic Depletion Kit

EasySep® box

The EasySep® Human Whole Blood CD45 Depletion Kit is designed to deplete CD45+ cells from fresh whole blood. Cells are targeted with Tetrameric Antibody Complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except on erythrocytes and platelets.

Product Name	Description	Catalog #	Size	Price	Quantity
EasySep® Human Whole Blood CD45 Depletion Kit	Immunomagnetic depletion of human CD45+ cells from whole blood	18289	For labeling 100 mL of whole blood	507.00 USD		Save Item
RoboSep® Human Whole Blood CD45 Depletion Kit with Filter Tips	EasySep® kit with RoboSep® buffer and RoboSep® filter tips	18289RF	For labeling 100 mL of whole blood	568.00 USD		Save Item

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Overview
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Data

Recommended for:	The depletion of human CD45+ cells from whole blood
Components:	• EasySep® Human Whole Blood CD45 Depletion Cocktail, 1.0 mL • EasySep® Magnetic Particles, 1.0 mL • HetaSep™, 20 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• “The Big Easy” EasySep® Magnet (Catalog #18001), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Area of Interest:	Cancer, Hematopoietic stem cell research, Immunology, Stem cell biology
Cell Isolation Product Type:	Reagents
Cell Source:	Whole blood
Cell Type:	Hematopoietic stem & progenitor cells, Lymphocytes, Tumor cells
Popular Product Line:	EasySep
Selection Method:	Depletion, Negative
Species:	Human

Procedures and instruction manuals:

PRODUCT INFORMATION SHEET - 18289, Lot# All:29037-PIS_1_0_0.pdf

Educational resources:

MSDS:

FAQS:

Q. HOW DOES THE SEPARATION WORK?

A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
Q. WHICH COLUMNS DO I USE?

A. None, columns are not used in the EasySep^® procedure.
Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

A. Yes, but this may affect separation performance.
Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

A. No, but they are so tiny, they do not interfere with downstream applications.
Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?

A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

A. Yes, we have products for both.
Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

A. Each Product Information Sheet has detailed staining information.
Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

A. No, we have found that cells are not functionally altered.
Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?

A. Yes, click here for a link to RoboSep^®.

This product has been used in:

Mattias Carlsten et al. Primary human tumor cells expressing CD155 impair tumor targeting by down-regulating DNAM-1 on NK cells.J Immunol 183 (8) 4921-4930 (October 15, 2009)

Product Name	Description	Catalog #
Ammonium Chloride Solution	Reagent for the Lysis of Red Blood Cells	07800
RoboSep® Red Blood Cell Lysis Buffer	Red Blood Cell Lysis Buffer	20110
CD45 Antibody, Clone 2D1, FITC-Conjugated	Mouse Monoclonal Antibody to Human CD45 - FITC Conjugated	10417
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000
HetaSep™	For Isolating Human Nucleated Cells from Peripheral Blood	07806
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000

FACS Profile Results With EasySep® Human Whole Blood CD45 Cell Depletion Kit

Starting with whole blood, the depletion of CD45+ cells ranges from 2-4 log. Plots were gated on Glycophorin A negative cells to exclude residual red blood cells. † 5E11 is an antibody against an epithelial cell surface antigen expressed on CAMA cells.