EasySep® Human T Cell Enrichment Kit

Recommended for:	The isolation of human T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:	• EasySep® Human T Cell Enrichment Cocktail, 1.0 mL • EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• EasySep® Magnet (Catalog #18000), or • “The Big Easy” EasySep® Magnet (Catalog #18001), or • RoboSep® (Catalog #20000), or • Easy 50 EasySep® Magnet (Catalog #18002)
Product Type:	Cell isolation products
Area of Interest:	HIV, Immunology
Cell Isolation Product Type:	Reagents
Cell Source:	Leukapheresis, PBMC
Cell Type:	T cells
Popular Product Line:	EasySep, RoboSep
Selection Method:	Negative
Species:	Human

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 19051, Lot# 10F35432 or less:28741-PIS_3_0_1.pdf
•  PRODUCT INFORMATION SHEET - 19051, Lot# 10J36489 or greater:28741-PIS_4_2_0.pdf
•  PRODUCT INFORMATION SHEET - 19051, Lot# 10J36489 or greater:28741-Easy50-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19051, Lot# 10J36489 or greater:28741-EasyPlate-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19051RF, Lot# All:28741-PIS_4_2_0.pdf

Educational resources:

•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: RoboSep®: Solutions for HIV Research
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  WALLCHART: Antigen Processing and Presentation Wallchart
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  WALLCHART: Frequencies of Cell Types in Human Peripheral Blood Wallchart

MSDS:

•  19011C2_19051C2-MSDS
•  19250_19250H-MSDS.pdf
•  19011HC1_19051HC1-MSDS

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Soumika Biswas et al. Elevated levels of select gangliosides in T cells from renal cell carcinoma patients is associated with T cell dysfunction.J Immunol 183 (8) 5050-5058 (October 15, 2009)
2. Jeffrey Milush et al. Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by co-expression of CD56, CD7, and CD4Blood 114 (23) 4823-4831 (October 5, 2009)
3. M W Pennington et al. Engineering a stable and selective peptide blocker of the Kv1.3 channel in T lymphocytes.Mol Pharmacol 75 (4) 762-773 (April 2009)
4. V Decot et al. Chimerism analysis following nonmyeloablative stem cell transplantation using a new cell subset separation method: Robosep.Biomed Mater Eng 18 (1 Suppl) S19-26 (2008)
5. Enrico Ammirati et al. Expansion of T-cell receptor zeta dim effector T cells in acute coronary syndromes.Arterioscler Thromb Vasc Biol 28 (12) 2305-2311 (December 2008)
6. Lesley White et al. Differential effects of IL-21 and IL-15 on perforin expression, lysosomal degranulation, and proliferation in CD8 T cells of patients with human immunodeficiency virus-1 (HIV).Blood 109 (9) 3873-3880 (May 1, 2007)
7. Christine Beeton et al. Kv1.3 channels are a therapeutic target for T cell-mediated autoimmune diseases.Proc Natl Acad Sci U S A 103 (46) 17414-17419 (November 14, 2006)
8. Kumudha Balakrishnan et al. Forodesine, an inhibitor of purine nucleoside phosphorylase, induces apoptosis in chronic lymphocytic leukemia cells.Blood 108 (7) 2392-2398 (October 1, 2006)

Product Name	Description	Catalog #
CD3 Antibody, Clone SK7, PE-Conjugated	Mouse Monoclonal Antibody to Human CD3 - PE Conjugated	10502
CD3 Antibody, Clone SK7, FITC-Conjugated	Mouse Monoclonal Antibody to CD3 - FITC Conjugated	10402
Ficoll-Paque™ PLUS	Density Gradient Medium for the Isolation of Mononuclear Cells	07907
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000

FACS Histogram Results with EasySep® Human T Cell Enrichment Kit

Starting with previously frozen mononuclear cells, the CD3+ cell content of the enriched fraction typically ranges from 95% - 99%.

Typical EasySep® Procedure for Human Cells