EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion

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Immunomagnetic Negative Selection Kit

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  • Box top label for EasySep Human Progenitor Enrichment Kit with Platelet Depletion For labeling 1 x 10e9 cells
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The EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion is designed to isolate hematopoietic progenitor cells from fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which depletion of platelets, megakaryocytes and megakaryocytic progenitor cells is not desired, we recommend the EasySep™ Human Progenitor Cell Enrichment Kit (Catalog #19056), which does not contain anti-CD61.
Product Name Description Catalog # Size Price Quantity
EasySep™ Human Progenitor Enrichment Kit with Platelet Depletion Immunomagnetic isolation of untouched human hematopoietic progenitors 19356 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep™ Human Progenitor Enrichment with Platelet Depletion EasySep™ kit with RoboSep™ Buffer 2 and RoboSep™ filter tips 19356RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Recommended for:
The isolation of human hematopoietic progenitor cells from fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets by negative selection
Components:
• EasySep™ Human Progenitor Cell Enrichment Cocktail with Platelet Depletion, 1.0 mL
• EasySep™ Magnetic Particles, 2 x 1.0 mL
Accessory Products:
• MethoCult™ Optimum (Catalog #04034)
• Iscove's MDM with 25 mM Hepes (Catalog #36150)
 
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep™ Magnet (Catalog #18000), or
• "The Big Easy" EasySep™ Magnet (Catalog #18001), or
• RoboSep™ (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Hematopoietic stem cell research, Immunology, Stem cell biology
Cell Isolation Product Type: Reagents
Cell Source: Cord blood, PBMC
Cell Type: Hematopoietic cells, Hematopoietic stem & progenitor cells
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Product Name

Description

Catalog #
CD34 Antibody, Clone 8G12, FITC-Conjugated Mouse Monoclonal Antibody to Human CD34 - FITC Conjugated 10413
MethoCult® H4034 Optimum Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Human Cells 04034
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
CD34 Antibody, Clone 8G12, PE-Conjugated Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 10513
DNase I Solution (1 mg/mL) Deoxyribonuclease I Solution (1 mg/mL) 07900
RoboSep™ RoboSep™ - The Fully Automated Cell Separator 20000
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
CD34 Antibody, Clone 8G12, APC-Conjugated Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 10613

Starting with nucleated cells, the CD34+ cell content of the enriched fraction typically ranges from 50 - 75% depending on the quality of the start sample.


Starting with nucleated cells, the CD34+ cell content of the enriched fraction typically ranges from 50 - 75% depending on the quality of the start sample.
Note: CD34+ enrichment is dependent on the frequency of CD34+ cells in the start sample, which is variable between cord blood samples. Use of poor quality cord or frozen samples may result in lower CD34+ cell content of the enriched fraction.


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