EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit

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Immunomagnetic Positive Selection or Depletion Kit

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  • EasySep® box
  • Box top label for Human CD25 Positive Selection Kit For labeling 10e9 total cells (Depletion) and 2 x 10e9 cells (Positive Selection)
EasySep® box
The EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit is designed to isolate or deplete all CD25+ cells from fresh peripheral blood mononuclear cells. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD25 antigen is expressed on regulatory T cells and activated T and B cells.
Product Name Description Catalog # Size Price Quantity
EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit Immunomagnetic positive selection or depletion of human CD25+ cells 18251 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep™ Human Pan-CD25 Positive Selection Kit EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18251RF For labeling up to 1 x 10e9 total cells 625.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation or depletion of human CD25+ cells from fresh  peripheral blood mononuclear cells
Components:
• EasySep™ Human Pan-CD25 Positive Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Equipment Required:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001), or
• RoboSep™ (Catalog #20000)
Legal Statement:
N/A
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: CD4+ T cells, CD8+ T cells, Regulatory T cells, T cells
Popular Product Line: EasySep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
Complete Kit for Human CD4+CD25+ T Cells Immunomagnetic Positive Selection Kit 15862
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001

Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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