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EasySep™ Human Neutrophil Enrichment Kit

Immunomagnetic Negative Selection Kit

  • EasySep™ Box
  • Box top label for Human Neutrophil Enrichment Kit For labeling 10e9 total cells
EasySep™ Box
Product Catalog # Size Price
EasySep™ Human Neutrophil Enrichment Kit
Immunomagnetic isolation of untouched human neutrophils
19257 For labeling up to 1 x 10e9 cells 895.00 USD
RoboSep™ Human Neutrophil Enrichment Kit with Filter Tips
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
19257RF For labeling up to 1 x 10e9 cells 930.00 USD
 

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Quick Order

  • EasySep™ Human Neutrophil Enrichment Kit (19257)
    For labeling up to 1 x 10e9 cells
    895.00 USD

  • RoboSep™ Human Neutrophil Enrichment Kit with Filter Tips (19257RF)
    For labeling up to 1 x 10e9 cells
    930.00 USD

Description


The EasySep™ Human Neutrophil Enrichment Kit is designed to isolate neutrophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD9, CD19, CD36, CD56, glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The enrichment kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation or red blood cell lysis.
Product Type: Cell isolation products
Recommended For:
The isolation of neutrophils from a polymorphonuclear cell-rich fraction by negative selection
Components:
• EasySep™ Human Neutrophil Enrichment Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
Equipment Required:
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: PMNC
Cell Type: Granulocytes & subsets
Popular Product Line: EasySep™, RoboSep™
Selection Method: Negative
Species: Human
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Technical Resources


Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
EasySep™ Human Neutrophil Enrichment Kit All Product Information Sheet
RoboSep™ Human Neutrophil Enrichment Kit with Filter Tips All Product Information Sheet

MSDS

Educational Resources

Videos & Webinars

  • EasySep™: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
  • The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
  • The EasySep™ MultiStand: Four Samples, Four Separations, One Pour-off
  • RoboSep™ Product Tour
  • How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
  • RoboSep™ Sneak Preview
  • Brochures

  • Cell Separation Products for Autoimmunity Research
  • Tools For Your Immunology Research
  • Wallcharts

  • Frequencies of Cell Types in Human Peripheral Blood Wallchart

  • FAQS


    • HOW EASYSEP™ WORKS

      • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
        A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
      • Q. HOW DOES THE SEPARATION WORK?
        A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
      • Q. WHICH COLUMNS DO I USE?
        A. The EasySep™ procedure is column-free. That's right - no columns!
      • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
        A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
      • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
        A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
      • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
        A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

      EASYSEP™ MAGNETIC PARTICLES

      • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
        A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
      • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
        A. No, but due to the small size of these particles, they will not interfere with downstream applications.
      • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
        A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

        If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

      MODIFYING THE EASYSEP™ PROTOCOL

      • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
        A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
      • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
        A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
     

    Data


    FACS Profile Results Using EasySep™ Human Neutrophil Enrichment Kit


    FACS Profile Results Using EasySep™ Human Neutrophil Enrichment Kit
    Starting with fresh HetaSep™-treated blood, the neutrophil content of the enriched fraction typically ranges from 98% - 99%.


    Typical EasySep™ Procedure for Human Cells


    Typical EasySep™ Procedure for Human Cells



    Related Products


    Other Reagents and Materials


    Product Name

    Description

    Catalog #

    HetaSep™ For Isolating Human Nucleated Cells from Peripheral Blood 07806

    Antibodies


    Product Name

    Description

    Catalog #

    Anti-Human CD32 Antibody, Clone IV.3 Mouse Monoclonal Antibody Against Human CD32 60012
    Anti-Human CD45 Antibody, Clone HI30 Mouse Monoclonal Antibody Against Human, Chimpanzee CD45 60018
    Anti-Dextran Antibody, Clone DX1 Mouse Monoclonal Antibody Against Dextran 60026

    Equipment and Accessories


    Product Name

    Description

    Catalog #

    EasySep™ Magnet Immunomagnetic Column-Free Magnet 18000
    "The Big Easy" EasySep™ Magnet Immunomagnetic Column-Free Magnet 18001
    RoboSep™-S RoboSep™-S - The Fully Automated Cell Separator 21000

    Publications


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