EasySep® Human Naïve CD4+ T Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • Box top label for Human Naïve CD4+ T Cell Enrichment Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human Naive CD4+ T Cell Enrichment Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, glycophorin A and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Naïve CD4+ T Cell Enrichment Kit Immunomagnetic isolation of untouched human naive CD4+ T cells 19155 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep® Human Naïve CD4+ T Cell Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19155RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of naive human CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:
• EasySep® Human Naïve CD4+ T Cell Enrichment Cocktail, 1.0 mL
• Biotinylated Anti-CD45RO Antibody, 1.0 mL
• EasySep® Magnetic Particles, 3 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells, Other T cell subsets, CD4+ T cells
Popular Product Line: EasySep
Area of Interest: Immunology, HIV
Species: Human
Cell Source: PBMC
Selection Method: Negative
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Daniel O Griffin et al. Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+ CD27+ CD43+ CD70-.J Exp Med 208 (1) 67-80 (January 17, 2011)
  2. Zhi-Jian Ye et al. Generation and differentiation of IL-17-producing CD4+ T cells in malignant pleural effusion.J Immunol 185 (10) 6348-6354 (November 15, 2010)
  3. Xinming Su et al. Tumor Microenvironments Direct the Recruitment and Expansion of Human Th17 CellsJ Immunol 184 (3) 1630-1641 (February 1, 2010)
  4. Muzlifah Haniffa et al. Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation.J Exp Med 206 (2) 371-385 (February 16, 2009)
  5. Sook Kyung Chang et al. B Lymphocyte Stimulator Regulates Adaptive Immune Responses by Directly Promoting Dendritic Cell MaturationJ Immunol 180 (11) 7394-7403 (June 1, 2008)
  6. Elisabeth Billard et al. Brucella suis Prevents Human Dendritic Cell Maturation and Antigen Presentation through Regulation of Tumor Necrosis Factor Alpha SecretionInfect. Immun. 75 (10) 4980-4989 (October 1, 2007)
  7. Nicole L. Yonkers et al. TLR Ligand-Dependent Activation of Naive CD4 T Cells by Plasmacytoid Dendritic Cells Is Impaired in Hepatitis C Virus InfectionJ Immunol 178 (7) 4436-4444 (April 1, 2007)
  8. Wayne G Shreffler et al. The major glycoprotein allergen from Arachis hypogaea, Ara h 1, is a ligand of dendritic cell-specific ICAM-grabbing nonintegrin and acts as a Th2 adjuvant in vitro.J Immunol 177 (6) 3677-3685 (September 15, 2006)

Product Name

Description

Catalog #
CD45RO Antibody, Clone UCHL1, PE-Conjugated Mouse Monoclonal Antibody to Human CD45RO - PE Conjugated 10524
CD45RA Antibody, Clone L48, FITC-Conjugated Mouse Monoclonal Antibody to Human CD45RA - FITC Conjuguated 10418
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

Start: 16% CD4+CD45RA+CD45RO- Cells


FACs Plot



Enriched: 91.3% CD4+CD45RA+CD45RO- Cells


FACs Plot
Starting with previously frozen mononuclear cells, the naïve CD4+ T cell content of the enriched fraction typically ranges from 84 - 95%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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