EasySep® Human NK Cell Enrichment Kit

? 1.800.667.0322  • > Contact Us

Immunomagnetic Negative Selection Kit

Email  Print


Free from STEMCELL

  • EasySep® box
  • Box top label for Human NK Cell Enrichment Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human NK Cell Enrichment Kit is designed to isolate NK cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD4, CD14, CD19, CD20, CD36, CD66b, CD123, HLA-DR, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep® Human NK Cell Enrichment Kit Immunomagnetic isolation of untouched human NK cells 19055 For labeling up to 1 x 10e9 cells 668.00 USD      
 Save Item
RoboSep® Human NK Cell Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19055RF For labeling up to 1 x 10e9 cells 702.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human NK cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:
• EasySep® Human NK Cell Enrichment Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® (Catalog #18001), or
• "Easy 50" EasySep® Magnet (Catalog #18002), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HIV, Immunology
Cell Isolation Product Type: Reagents
Cell Source: Leukapheresis, PBMC
Cell Type: NK cells
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Giuliana Magri et al. NKp46 and DNAM-1 NK-cell receptors drive the response to human cytomegalovirus-infected myeloid dendritic cells overcoming viral immune evasion strategies.Blood 117 (3) 848-856 (January 20, 2011)
  2. Claudio Costantini et al. Neutrophil activation and survival are modulated by interaction with NK cells.Int Immunol 22 (10) 827-838 (October 2010)
  3. Holly M Horton et al. Fc-engineered anti-CD40 antibody enhances multiple effector functions and exhibits potent in vitro and in vivo antitumor activity against hematologic malignancies.Blood 116 (16) 3004-3012 (October 21, 2010)
  4. M Firoz Mian et al. FimH can directly activate human and murine natural killer cells via TLR4.Mol Ther 18 (7) 1379-1388 (July 2010)
  5. Aura Muntasell et al. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.Blood 115 (25) 5170-5179 (June 24, 2010)
  6. Jonathan Richard et al. HIV-1 Vpr up-regulates expression of ligands for the activating NKG2D receptor and promotes NK cell-mediated killing.Blood 115 (7) 1354-1363 (February 18, 2010)
  7. Courtney A Crane et al. TGF-beta downregulates the activating receptor NKG2D on NK cells and CD8+ T cells in glioma patients.Neuro Oncol 12 (1) 7-13 (January 2010)
  8. Claudio Costantini et al. On the co-purification of 6-sulfo LacNAc(+) dendritic cells (slanDC) with NK cells enriched from human blood.Immunobiology 214 (9-10) 828-834 (2009)
  9. Sara J Conry et al. Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.J Virol 83 (21) 11175-11187 (November 2009)
  10. Andr??s L??pez-Albaitero et al. Role of polymorphic Fc gamma receptor IIIa and EGFR expression level in cetuximab mediated, NK cell dependent in vitro cytotoxicity of head and neck squamous cell carcinoma cells.Cancer Immunol Immunother 58 (11) 1853-1864 (November 2009)
  11. K L Sand et al. Modulation of natural killer cell cytotoxicity and cytokine release by the drug glatiramer acetate.Cell Mol Life Sci 66 (8) 1446-1456 (April 2009)
  12. Manuela Fogli et al. Lysis of endogenously infected CD4+ T cell blasts by rIL-2 activated autologous natural killer cells from HIV-infected viremic individuals.PLoS Pathog 4 (7) e1000101 (July 2008)
  13. James Arthos et al. HIV-1 envelope protein binds to and signals through integrin [alpha]4[beta]7, the gut mucosal homing receptor for peripheral T cellsNat Immunol advanced online publication (3) 301-309 (February 10, 2008)
  14. Helene B. Bernstein et al. CD4 Expression on Activated NK Cells: Ligation of CD4 Induces Cytokine Expression and Cell MigrationJ Immunol 177 (6) 3669-3676 (September 15, 2006)

Product Name

Description

Catalog #
CD56 Antibody, Clone NCAM16.2, PE-Conjugated Mouse Monoclonal Antibody to Human - CD56 PE Conjugated 10526
CD3 Antibody, Clone SK7, PE-Conjugated Mouse Monoclonal Antibody to Human CD3 - PE Conjugated 10502
CD3 Antibody, Clone SK7, FITC-Conjugated Mouse Monoclonal Antibody to CD3 - FITC Conjugated 10402
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
Easy 50 EasySep® Magnet Immunomagnetic Column-Free Magnet 18002
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Profile Results With EasySep® Human NK Cell Enrichment Kit


FACS Profile Results With EasySep® Human NK Cell Enrichment Kit
The NK cell content of the enriched fraction varies, depending on the starting sample. Starting with previously frozen mononuclear cells containing more than 10% NK cells, the NK cell content of the enriched fraction typically ranges from 73% - 95%. Purities may be lower when starting with samples containing less than 10% NK cells.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



Recently Viewed Items