EasySep® Human Myeloid Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human Myeloid Cell Selection Kit For labeling 10e9 cells
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The EasySep® Human Myeloid Positive Selection Kit is designed to isolate CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with SpinSep® Density Medium (Catalog #17531) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens are expressed on myeloid progenitors monocytes and granulocytes.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Myeloid Positive Selection Kit Immunomagnetic positive selection of human CD33+ and CD66+ cells (e.g. myeloid cells) 18653 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep® Human Myeloid Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18653RF For labeling up to 1 x 10e9 cells 625.00 USD      
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Call us: +1.604.877.0713  
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Recommended for:
The isolation of human CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with SpinSep® Density Medium (Catalog #17531) by positive selection.
Components:
• EasySep® Human Myeloid Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
• SpinSep® Density Medium, 100 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Hematologic malignancies, Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: Myeloid cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
RosetteSep® Human Granulocyte Enrichment Cocktail Immunodensity Negative Selection Cocktail 15121
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
SpinSep® Density Medium 17531

FACS Histogram Results Using EasySep® Human Myeloid Cell Positive Selection Kit


FACS Histogram Results Using EasySep® Human Myeloid Cell Positive Selection Kit
Starting with fresh nucleated cells, the myeloid cell content of the enriched fraction typically ranges from 96.2% - 99.0%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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