EasySep® Human Monocyte Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • EasySep® box
  • Box top label for Human Monocyte Enrichment Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD16, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep® Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058).
Product Name Description Catalog # Size Price Quantity
EasySep® Human Monocyte Enrichment Kit Immunomagnetic isolation of untouched human monocytes 19059 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep® Human Monocyte Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19059RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:
• EasySep® Human Monocyte Enrichment Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HIV, Immunology
Cell Isolation Product Type: Reagents
Cell Source: Leukapheresis, PBMC
Cell Type: Monocytes
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Ana Merino et al. Senescent CD14+ CD16+ monocytes exhibit proinflammatory and proatherosclerotic activity.J Immunol 186 (3) 1809-1815 (February 1, 2011)
  2. Carole Berger et al. Rapid generation of maturationally synchronized human dendritic cells: contribution to the clinical efficacy of extracorporeal photochemotherapy.Blood 116 (23) 4838-4847 (December 2, 2010)
  3. Vinod S Ramgolam et al. IFN-beta inhibits human Th17 cell differentiation.J Immunol 183 (8) 5418-5427 (October 15, 2009)

Product Name

Description

Catalog #
CD14 Antibody, Clone MoP9, PE-Conjugated Mouse Monoclonal Antibody to Human CD14 - PE Conjugated 10506
CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406
Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
Easy 50 EasySep® Magnet Immunomagnetic Column-Free Magnet 18002
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Profile Results Using EasySep® Human Monocyte Enrichment Kit


FACS Profile Results Using EasySep® Human Monocyte Enrichment Kit
Starting with previously frozen peripheral blood mononuclear cells, the monocyte content of the enriched fraction typically ranges from 83% - 95%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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