EasySep® Human Monocyte Enrichment Kit without CD16 Depletion

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Immunomagnetic Negative Selection Kit

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  • Box top label for Human Monocyte Enrichment Kit without CD16 Depletion For labeling 10e9 cells
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The EasySep® Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep® Human Monocyte Enrichment Kit (Catalog #19059), which contains anti-CD16.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Monocyte Enrichment Kit without CD16 Depletion Immunomagnetic isolation of untouched human monocytes 19058 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep® Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19058RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
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Recommended for:
The isolation of human monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:
• EasySep® Human Monocyte Enrichment Cocktail without CD16 Depletion, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• "Easy 50" EasySep® Magnet (Catalog #18002), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HIV, Immunology
Cell Isolation Product Type: Reagents
Cell Source: Leukapheresis, PBMC
Cell Type: Monocytes
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Carolina Garc??a-de-Alba et al. Expression of Matrix Metalloproteases by Fibrocytes. Possible Role in Migration and Homing.Am J Respir Crit Care Med (July 9, 2010)
  2. Shanshan Lin et al. HIV infection upregulates Caveolin 1 (Cav-1) expression to restrict virus production.J Virol (July 7, 2010)
  3. Nicholas J Megjugorac et al. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.Blood 115 (21) 4185-4190 (May 27, 2010)
  4. Martin Pelletier et al. Evidence for a cross-talk between human neutrophils and Th17 cells.Blood 115 (2) 335-343 (January 14, 2010)
  5. Ryan A Wilcox et al. Monocytes promote tumor cell survival in T-cell lymphoproliferative disorders and are impaired in their ability to differentiate into mature dendritic cells.Blood 114 (14) 2936-2944 (October 1, 2009)

Product Name

Description

Catalog #
CD14 Antibody, Clone MoP9, PE-Conjugated Mouse Monoclonal Antibody to Human CD14 - PE Conjugated 10506
CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406
Ficoll-Paque™ PREMIUM Density Gradient Medium for the Isolation of Mononuclear Cells 07908
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
Easy 50 EasySep® Magnet Immunomagnetic Column-Free Magnet 18002
EasySep® Human Monocyte Enrichment Kit Immunomagnetic Negative Selection Kit 19059
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results Using EasySep® Human Monocyte Enrichment Kit Without CD16 Depletion


FACS Histogram Results Using EasySep® Human Monocyte Enrichment Kit Without CD16 Depletion
Starting with freshly prepared peripheral blood mononuclear cells, the CD14+ cell content of the enriched fraction typically ranges from 73% - 81%. Slightly lower CD14+ cell purities may be obtained from samples that contain a large number of CD16+ cells.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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