EasySep™ Human Memory CD8+ T Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • EasySep® box
  • Box top label for EasySep Human Memory CD8+ T Cell Enrichment Kit For labeling 10e9 cells
  • Box top label for RoboSep Human Memory CD8+ T Cell Enrichment Kit For labeling 10e9 cells
EasySep® box
The EasySep™ Human Memory CD8+ T Cell Enrichment Kit is designed to isolate memory CD8+ T cells from peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD4, CD14, CD16, CD19, CD20, CD34, CD36, CD45RA, CD56, CD61, CD66b, CD123, TCRγ/δ, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep™ Human Memory CD8+ T Cell Enrichment Kit Immunomagnetic isolation of untouched human memory CD8+ T cells 19159 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep™ Human Memory CD8+ T Cell Enrichment Kit EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 19159RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human memory CD8+ T cells from peripheral blood mononuclear cells by negative selection
Components:
• EasySep™ Memory CD8+ T Cell Enrichment Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001), or
• RoboSep™ (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Leukapheresis, PBMC
Cell Type: CD8+ T cells, T cells
Popular Product Line: EasySep
Selection Method: Negative
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Product Name

Description

Catalog #
CD45RA Antibody, Clone L48, FITC-Conjugated Mouse Monoclonal Antibody to Human CD45RA - FITC Conjuguated 10418
CD45RO Antibody, Clone UCHL1, PE-Conjugated Mouse Monoclonal Antibody to Human CD45RO - PE Conjugated 10524
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000

Start: 4.9% CD8+CD45RA-CD45RO+ of total peripheral blood mononuclear cells (PBMC)


Start: 4.9% CD8+CD45RA-CD45RO+ of total peripheral blood mononuclear cells (PBMC)



Enriched: 88.8% CD8+CD45RA-CD45RO+ of enriched fraction


Enriched: 88.8% CD8+CD45RA-CD45RO+ of enriched fraction
Starting with mononuclear cells, the memory CD8+ T cell content of the enriched fraction typically ranges from 72 - 92%.


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