EasySep® Human Memory CD4+ T Cell Enrichment Kit

Recommended for:	The isolation of human memory CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:	• EasySep® Human Memory CD4+ T Cell Enrichment Cocktail, 1.0 mL • EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• EasySep® Magnet (Catalog #18000), or • “The Big Easy” EasySep® Magnet (Catalog #18001), or • "Easy 50" EasySep® Magnet (Catalog #18002), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Area of Interest:	HIV, Immunology
Cell Isolation Product Type:	Reagents
Cell Source:	PBMC
Cell Type:	CD4+ T cells, Other T cell subsets, T cells
Popular Product Line:	EasySep
Selection Method:	Negative
Species:	Human

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 19157, Lot# All:28945-PIS_2_3_0.pdf
•  PRODUCT INFORMATION SHEET - 19157, Lot# All:28945-EasyPlate-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19157, Lot# All:28945-Easy50-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19157RF, Lot# All:28945-PIS_2_3_0.pdf

Educational resources:

•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: Cell Separation for Autoimmunity Research
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  SCIENTIFIC POSTER: Single-step isolation of highly purified, untouched human CD4+ memory T cells by column-free immunomagnetic cell separation
•  WALLCHART: Antigen Processing and Presentation Wallchart
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  WALLCHART: Frequencies of Cell Types in Human Peripheral Blood Wallchart

MSDS:

•  19117C_19157C-MSDS.pdf
•  19250_19250H-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

Product Name	Description	Catalog #
CD45RO Antibody, Clone UCHL1, PE-Conjugated	Mouse Monoclonal Antibody to Human CD45RO - PE Conjugated	10524
CD45RA Antibody, Clone L48, FITC-Conjugated	Mouse Monoclonal Antibody to Human CD45RA - FITC Conjuguated	10418
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
Easy 50 EasySep® Magnet	Immunomagnetic Column-Free Magnet	18002
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000

Typical Enrichment Profile For EasySep® Human Memory CD4+ T Cell Enrichment Kit

Starting with previously frozen mononuclear cells, the memory CD4+ T cell content of the enriched fraction typically ranges from 86% - 98%.

Typical EasySep® Procedure for Human Cells