EasySep® Human EpCAM Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human EpCAM Positive Selection Kit For labeling 10e9 cells
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The EasySep® Human EpCAM Positive Selection Kit is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.
Product Name Description Catalog # Size Price Quantity
EasySep® Human EpCAM Positive Selection Kit Immunomagnetic positive selection of human EpCAM+ cells (e.g. mammary epithelial cells) 18356 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep® Human EpCAM Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18356RF For labeling up to 1 x 10e9 cells 625.00 USD      
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Recommended for:
The isolation of human EpCAM+ cells from fresh or previously frozen mammary epithelial cell preparations by positive selection
Components:
• EasySep® Human EpCAM Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Cancer, Mammary cell research
Cell Isolation Product Type: Reagents
Cell Source: Other
Cell Type: Mammary epithelial cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

This product has been used in:

  1. Kevin G Leong et al. Jagged1-mediated Notch activation induces epithelial-to-mesenchymal transition through Slug-induced repression of E-cadherin.J Exp Med 204 (12) 2935-2948 (November 26, 2007)

Product Name

Description

Catalog #
EpCAM Antibody, Clone VU-1D9, FITC-Conjugated Mouse Monoclonal Antibody to Human Epithelial Cell Adhesion Molecule (EpCAM) - FITC Conjugated 10109
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep™ RoboSep™ - The Fully Automated Cell Separator 20000
MammoCult® Human Medium Kit Medium for the Culture of Human Mammospheres and Tumorspheres 05620

FACS Histogram Results With EasySep® Human EpCAM Selection Kit


FACS Histogram Results With EasySep® Human EpCAM Selection Kit
Starting with cultured mammary tissue, the typical purity of the selected fraction is 93 ± 1%. Purity has been assessed by staining with the monoclonal antibody (5E11-FITC, Catalog #10110) which has a distribution identical to EpCAM.


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