EasySep® Human "Do-It-Yourself" Selection Kit

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Immunomagnetic Positive Selection Kit

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  • EasySep® box
  • Box top label for Human "Do-It-Yourself" Selection Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human "Do-It-Yourself" Selection Kit is designed to select any human cell type of interest with your own mouse IgG1 monoclonal antibody. Cells that express the surface antigen that is recognized by the mouse IgG1 monoclonal antibody are targeted for magnetic separation using EasySep® Magnetic Particles and the EasySep® Magnet. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding.
Product Name Description Catalog # Size Price Quantity
EasySep® Human "Do-It-Yourself" Selection Kit Immunomagnetic positive selection of any human cells with your own mouse IgG1 antibody 18099 For labeling up to 1 x 10e9 cells 459.00 USD      
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RoboSep® Human Do-it-Yourself Selection with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18099RF For labeling up to 1 x 10e9 cells 492.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of any human cells using your own mouse IgG1 antibody by positive selection
Components:
• EasySep® “Do-It-Yourself” Component A
• EasySep® “Do-It-Yourself” Component B
• EasySep® “Do-It-Yourself” Component C
• EasySep® Magnetic Particles
• RoboSep® Vial for “Do-It-Yourself” Antibody Cocktail (not required for manual use)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HIV, Immunology
Cell Isolation Product Type: Custom isolation, Reagents
Cell Source: PBMC
Cell Type: Other
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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