EasySep® Human CD56 Positive Selection Kit

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 18055, Lot# 09F30615 or greater:28385-PIS_2_2_0.pdf
•  PRODUCT INFORMATION SHEET - 18055RF, Lot# All:28385-PIS_2_2_0.pdf

Educational resources:

•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: Cell Separation Products for HLA Analysis
•  BROCHURE: RoboSep®: Solutions for HIV Research
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  WEBINAR: Cell Separation Solutions for HLA and Chimerism Analysis
•  VIDEO: RosetteSep®: A Guide to Isolating NK Cells Directly from Whole Blood
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  BROCHURE: Fast and Easy Isolation of Natural Killer Cells
•  BROCHURE: Fully Automated, High Purity Cell Isolation for Chimerism Labs
•  WALLCHART: Frequencies of Cell Types in Human Peripheral Blood Wallchart

MSDS:

•  18150_18150H-MSDS.pdf
•  18015C.2_18055C.2-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
  
  A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
• 
  Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?
  
  A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
• 
  Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?
  
  A. No, but they are so tiny, they do not interfere with downstream applications.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
  
  A. No, we have found that cells are not functionally altered.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Nicholas J Megjugorac et al. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.Blood 115 (21) 4185-4190 (May 27, 2010)
2. Nicholas Watkins et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells.Blood 113 (19) e1-9 (May 7, 2009)
3. Hisaki Fujii et al. In vivo control of acute lymphoblastic leukemia by immunostimulatory CpG oligonucleotides.Blood 109 (5) 2008-2013 (March 1, 2007)
4. Donald N. Forthal et al. Interactions between natural killer cells and antibody Fc result in enhanced antibody neutralization of human immunodeficiency virus type 1.J. Virol. 79 (4) 2042-2049 (February 15, 2005)
5. Matthew Bonaparte et al. Killing of human immunodeficiency virus-infected primary T-cell blasts by autologous natural killer cells is dependent on the ability of the virus to alter the expression of major histocompatibility complex class I moleculesBlood 104 (7) (October 1, 2004)

FACS Histogram Results with EasySep® Human CD56+ Positive Selection Kit

Typical EasySep® Procedure for Human Cells