EasySep® Human CD4+CD127low T Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • Box top label for Human CD4+CD127low T Cell Enrichment Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human CD4+CD127low T Cell Enrichment Kit is designed to isolate CD4+CD127low T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high cells, and dextran-coated magnetic particles. The labeled cells are separated using the EasySep® magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD4+CD127low T Cell Enrichment Kit Immunomagnetic isolation of untouched human CD4+CD127low T cells 19231 For labeling up to 1 x 10e9 cells 668.00 USD      
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RoboSep® Human CD4+CD127low T Cell Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19231RF For labeling up to 1 x 10e9 cells 702.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD4+CD127low T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:
• EasySep® Human CD4+CD127low T Cell Enrichment Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells, Regulatory T cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology
Species: Human
Cell Source: PBMC
Selection Method: Negative
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
CD3 Antibody, Clone SK7, FITC-Conjugated Mouse Monoclonal Antibody to CD3 - FITC Conjugated 10402
CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406
CD16 Antibody, Clone NKP15, FITC-Conjugated Mouse Monoclonal Antibody to Human CD16 - FITC Conjugated 10408
CD19 Antibody, Clone 4G7, FITC-Conjugated Mouse Monoclonal Antibody to Human CD19 - FITC Conjugated 10409
CD20 Antibody, Clone L27, FITC-Conjugated Mouse Monoclonal Antibody to Human CD20 - FITC Conjugated 10410
CD34 Antibody, Clone 8G12, FITC-Conjugated Mouse Monoclonal Antibody to Human CD34 - FITC Conjugated 10413
CD56 Antibody, Clone NCAM16.2, PE-Conjugated Mouse Monoclonal Antibody to Human - CD56 PE Conjugated 10526
CD123/IL-3 R-alpha Antibody, Clone 9F5, PE-Conjugated Mouse Monoclonal Antibody to Human CD123 (IL-3 Rα) - PE Conjugated 10532
StemSpan® SFEM Serum-Free Medium for Expansion of Hematopoietic Cells 09600
StemSpan® SFEM 09650

FACS Profile Results With EasySep® Human CD4+CD127low T Cell Enrichment Kit


FACS Profile Results With EasySep® Human CD4+CD127low T Cell Enrichment Kit
Starting with peripheral blood mononuclear cells, the CD4+CD127low cell content of the enriched fraction typically ranges from 86% - 97%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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