EasySep® Human CD45 Depletion Kit

? 1.800.667.0322  • > Contact Us

Immunomagnetic Depletion Kit

Email  Print


Free from STEMCELL

  • Test Tube Box EasySep®
  • Box top label for Human CD45 Depletion Kit For labeling up to 2 x 10e9 cells
Test Tube Box EasySep®
The EasySep® Human CD45 Depletion Kit is designed to deplete CD45+ cells from ammonium chloride lysed blood or bone marrow, or from fresh or previously frozen human peripheral blood mononuclear cells. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD45 Depletion Kit Immunomagnetic depletion of human CD45+ cells 18259 For labeling up to 2 x 10e9 cells 592.00 USD      
 Save Item
RoboSep® Human CD45 Depletion Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18259RF For labeling up to 2 x 10e9 cells 625.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The depletion of human CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells
Components:
• EasySep® Human CD45 Depletion Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Cancer, Hematopoietic stem cell research, Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: Tumor cells
Popular Product Line: EasySep, RoboSep
Selection Method: Depletion
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

This product has been used in:

  1. Paulo Navarro-Costa et al. Incorrect DNA methylation of the DAZL promoter CpG island associates with defective human sperm.Hum Reprod 25 (10) 2647-2654 (October 2010)
  2. Alberto Fusi et al. Expression of the Stem Cell Markers Nestin and CD133 on Circulating Melanoma Cells.J Invest Dermatol (September 30, 2010)
  3. A Fusi et al. Monitoring of circulating tumor cells in a patient with synchronous metastatic melanoma and colon carcinoma.Ann Oncol 21 (8) 1734-1735 (August 2010)
  4. Mattias Carlsten et al. Primary human tumor cells expressing CD155 impair tumor targeting by down-regulating DNAM-1 on NK cells.J Immunol 183 (8) 4921-4930 (October 15, 2009)
  5. Patricia Landry et al. Existence of a microRNA pathway in anucleate platelets.Nat Struct Mol Biol 16 (9) 961-966 (September 2009)
  6. Amy E Anderson et al. LPS activation is required for migratory activity and antigen presentation by tolerogenic dendritic cells.J Leukoc Biol 85 (2) 243-250 (February 2009)
  7. Liying Yang et al. Optimization of an enrichment process for circulating tumor cells from the blood of head and neck cancer patients through depletion of normal cells.Biotechnol Bioeng 102 (2) 521-534 (February 1, 2009)
  8. Mattias Carlsten et al. DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells.Cancer Res 67 (3) 1317-1325 (February 1, 2007)
  9. Dolores Baksh et al. Adult human bone marrow-derived mesenchymal progenitor cells are capable of adhesion-independent survival and expansion.Exp Hematol 31 (8) 723-732 (August 2003)

Product Name

Description

Catalog #
CD45 Antibody, Clone 2D1, FITC-Conjugated Mouse Monoclonal Antibody to Human CD45 - FITC Conjugated 10417
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep™ RoboSep™ - The Fully Automated Cell Separator 20000
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800

FACS Profile Results With EasySep® Human CD45 Cell Depletion Kit


FACS Profile Results With EasySep® Human CD45 Cell Depletion Kit
Starting with whole blood, the depletion of CD45+ cells is typically 4.0 log. Plots were gated on Glycophorin A negative cells to exclude residual red blood cells. Ber-EP4 is an antibody against an epithelial cell surface antigen.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



Recently Viewed Items