EasySep® Human CD4 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human CD4 Positive Selection Kit For labeling 10e9 cells
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The EasySep® Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD4 Positive Selection Kit Immunomagnetic positive selection of human CD4+ cells (e.g. CD4+ T cells) 18052 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep® Human CD4 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18052RF For labeling up to 1 x 10e9 cells 625.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection
Components:
• EasySep® Human CD4 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells, CD4+ T cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology, HIV
Species: Human
Cell Source: PBMC
Selection Method: Positive
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Karrie K Wong et al. The role of CD200 in immunity to B cell lymphoma.J Leukoc Biol (May 4, 2010)
  2. Daniela Fenoglio et al. Vdelta1 T lymphocytes producing IFN-gamma and IL-17 are expanded in HIV-1-infected patients and respond to Candida albicans.Blood 113 (26) 6611-6618 (June 25, 2009)
  3. Nicholas Watkins et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells.Blood 113 (19) e1-9 (May 7, 2009)
  4. Helene B Bernstein et al. CD4 expression on activated NK cells: ligation of CD4 induces cytokine expression and cell migration.J Immunol 177 (6) 3669-3676 (September 15, 2006)
  5. Jian Li et al. Human antibodies for immunotherapy development generated via a human B cell hybridoma technology.Proc Natl Acad Sci U S A 103 (10) 3557-3562 (March 7, 2006)
  6. Donald N. Forthal et al. Interactions between natural killer cells and antibody Fc result in enhanced antibody neutralization of human immunodeficiency virus type 1.J. Virol. 79 (4) 2042-2049 (February 15, 2005)

Product Name

Description

Catalog #
CD4 Antibody, Clone L120, PE-Conjugated Mouse Monoclonal Antibody to Human CD4 - PE Conjugated 10435
CD4 Antibody, Clone L120, FITC-Conjugated Mouse Monoclonal Antibody to Human CD4 - FITC Conjugated 10434
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results Using EasySep® Human CD4 Positive Selection Kit


FACS Histogram Results Using EasySep® Human CD4 Positive Selection Kit
Starting with monocyte-depleted fresh peripheral blood mononuclear cells, the CD4+ T cell content of the selected fraction typically ranges from 97.4% - 99.5%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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