EasySep® Human CD34 Positive Selection Kit

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 18056, Lot# All:28386-PIS_2_1_1.pdf
•  PRODUCT INFORMATION SHEET - 18056RF, Lot# All:28386-PIS_2_1_1.pdf

Educational resources:

•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  WEBINAR: Cell Separation Solutions for HLA and Chimerism Analysis
•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: Cell Separation Products for HLA Analysis
•  BROCHURE: The World Leader in Tools For Hematopoietic Stem Cell Research
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  SCIENTIFIC POSTER: Isolation and enrichment of rare cells with an imunomagnetic cell selection system
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  BROCHURE: Fully Automated, High Purity Cell Isolation for Chimerism Labs
•  WALLCHART: Frequencies of Cell Types in Human Peripheral Blood Wallchart

MSDS:

•  18016C.1_18056C.1-MSDS.pdf
•  18150_18150H-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
  
  A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
• 
  Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?
  
  A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
• 
  Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?
  
  A. No, but they are so tiny, they do not interfere with downstream applications.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
  
  A. No, we have found that cells are not functionally altered.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Leslie J Marshall et al. Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity.J Gen Virol 91 (Pt 12) 3042-3052 (December 2010)
2. Elisa Bianchi et al. c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression.Blood 116 (22) e99-110 (November 25, 2010)
3. Xiaoyan Jiang et al. Properties of CD34+ CML stem/progenitor cells that correlate with different clinical responses to imatinib mesylate.Blood (June 23, 2010)
4. Christoph Carter et al. HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirsNat Med advance online publication (4) 446-451 (March 7, 2010)
5. Xiaoli Wang et al. Correction of the Abnormal Trafficking of Primary Myelofibrosis CD34+ Cells by Treatment with Chromatin-Modifying AgentsCancer Res 0008-5472.CAN-09-1823 (September 14, 2009)
6. Jamshid Sorouri Khorashad et al. The level of BCR-ABL1 kinase activity before treatment does not identify chronic myeloid leukemia patients who fail to achieve a complete cytogenetic response on imatinib.Haematologica 94 (6) 861-864 (June 2009)
7. Tomasz Stoklosa et al. BCR/ABL inhibits mismatch repair to protect from apoptosis and induce point mutations.Cancer Res 68 (8) 2576-2580 (April 15, 2008)
8. Leman Yalcintepe et al. Expression of interleukin-3 receptor subunits on defined subpopulations of acute myeloid leukemia blasts predicts the cytotoxicity of diphtheria toxin interleukin-3 fusion protein against malignant progenitors that engraft in immunodeficient mice.Blood 108 (10) 3530-3537 (November 15, 2006)
9. Mark Wunderlich et al. Human CD34+ cells expressing the inv(16) fusion protein exhibit a myelomonocytic phenotype with greatly enhanced proliferative ability.Blood 108 (5) 1690-1697 (September 1, 2006)
10. Inge Hoebeke et al. Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hematopoietic stem cells.Blood 107 (7) 2879-2881 (April 1, 2006)
11. Petter S Woll et al. Human embryonic stem cell-derived NK cells acquire functional receptors and cytolytic activity.J Immunol 175 (8) 5095-5103 (October 15, 2005)
12. Andres Hidalgo et al. Enforced fucosylation of neonatal CD34+ cells generates selectin ligands that enhance the initial interactions with microvessels but not homing to bone marrow.Blood 105 (2) 567-575 (January 15, 2005)
13. Suzan Imren et al. High-level beta-globin expression and preferred intragenic integration after lentiviral transduction of human cord blood stem cells.J Clin Invest 114 (7) 953-962 (October 2004)
14. Cyrile A Curat et al. From blood monocytes to adipose tissue-resident macrophages: induction of diapedesis by human mature adipocytes.Diabetes 53 (5) 1285-1292 (May 2004)
15. Albertus W Wognum et al. Identification and isolation of hematopoietic stem cells.Arch Med Res 34 (6) 461-475

FACS Profile Results with EasySep® Human CD34+ Positive Selection Kit

Typical EasySep® Procedure for Human Cells