EasySep® Human CD3 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human CD3 Positive Selection Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human CD3 Positive Selection Kit is designed to isolate CD3+ T cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on all T cells and CD56+ NKT cells.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD3 Positive Selection Kit Immunomagnetic positive selection of human CD3+ cells (e.g. T cells) 18051 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep® Human CD3 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18051RF For labeling up to 1 x 10e9 cells 625.00 USD      
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Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD3+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection
Components:
• EasySep® Human CD3+ T Cell Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology, HIV
Species: Human
Cell Source: PBMC, Other
Selection Method: Positive
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
CD3 Antibody, Clone SK7, PE-Conjugated Mouse Monoclonal Antibody to Human CD3 - PE Conjugated 10502
CD3 Antibody, Clone SK7, FITC-Conjugated Mouse Monoclonal Antibody to CD3 - FITC Conjugated 10402
Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
EasySep® HLA CD3 Positive Selection Kit Immunomagnetic Positive Selection Kit 18051HLA
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results with EasySep® Human CD3 Positive Selection Kit


FACS Histogram Results with EasySep® Human CD3 Positive Selection Kit
Starting with fresh peripheral blood mononuclear cells, the CD3+ cell content of the selected fraction typically ranges from 99.4% - 99.8%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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