EasySep® Human CD271 Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Test Tube Box EasySep®
  • Box top label for Human CD271 Positive Selection Kit For labeling up to 2 x 10e9 cells
Test Tube Box EasySep®
The EasySep® Human CD271 Positive Selection Kit is designed to isolate CD271+ cells from fresh human bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD271 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD271+ antigen is expressed on monocytes and macrophages.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD271 Selection Kit Immunomagnetic positive selection of human CD271+ cells 18659 For labeling up to 2 x 10e9 cells 534.00 USD      
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Recommended for:
The isolation of human CD271+ cells from fresh bone marrow by positive selection
Components:
• EasySep® Human CD271 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
• EasySep® Human FcR Blocker, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
EasySep® Magnet (Catalog #18000)
Product Type: Cell isolation products
Area of Interest: Mesenchymal stem cell research
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Other
Cell Type: Mesenchymal stem cells
Popular Product Line: EasySep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
MesenCult®-XF Culture Kit Defined, Xeno-Free Culture Kit for Human Mesenchymal Stem Cells 05429
MesenCult®-XF Medium Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells 05420
MesenCult® Proliferation Kit (Human) Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells 05411
Fetal Bovine Serum for Human Mesenchymal Stem Cells Prescreened FBS for human mesenchymal stem cells 06471
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001

Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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