EasySep® Human CD25 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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Free from STEMCELL

  • Box top label for Human CD25 Positive Selection Kit For labeling up to 8 x 10e8 cells
Box top label for Human CD25 Positive Selection Kit For labeling up to 8 x 10e8 cells
The EasySep® Human CD25 Positive Selection Kit is designed to isolate CD25+ cells from CD4+ T cell or regulatory T cell enriched samples by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD25 antigen is expressed at varying levels on human regulatory T cells, activated T cells, B cells and monocytes.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD25 Positive Selection Kit Immunomagnetic positive selection of human CD25high cells 18231 For labeling up to 8 x 10e8 total cells 209.00 USD      
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Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Recommended for:
EasySep® Positive selection of human CD25+ cells following CD4+ enrichment by EasySep® (Catalog #19052) or RosetteSep® (Catalog #15022) or Regulatory T Cell Pre-Enrichment (Catalog #15321 and 15324)
Components:
• EasySep® Human CD25 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Accessory Products:
• EasySep® CD4+ T Cell Enrichment (Catalog #19052)
• RosetteSep CD4+ T Cell Enrichment (Catalog #15022)
• RosetteSep CD4+CD127low Regulatory T Cell Pre-Enrichment (Catalog #15321)
• RosetteSep CD4+CD127lowCD49d- Regulatory T Cell Pre-Enrichment (Catalog #15324)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• "The Big Easy" EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000).
Product Type: Cell isolation products
Cell Type: T cells, Regulatory T cells
Popular Product Line: EasySep
Area of Interest: Immunology
Species: Human
Cell Source: PBMC
Selection Method: Positive
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
Complete Kit for Human CD4+CD25+ T Cells Immunomagnetic Positive Selection Kit 15862
EasySep® Human CD4+CD127low T Cell Enrichment Kit Immunomagnetic Negative Selection Kit 19231
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells Immunomagnetic Positive Selection Kit 15861
Complete Kit for Human CD4+CD127lowCD49d-CD25high Regulatory T Cells Immunomagnetic Positive Selection Kit 15864
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001

Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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