EasySep™ Human CD19 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

  • EasySep™ Box
  • Box top label for Human CD19 Positive Selection Kit For labeling 10e9 cells
EasySep™ Box
The EasySep™ Human CD19 Positive Selection Kit is designed to isolate CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is expressed on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.
Product Name Description Catalog # Size Price Quantity
EasySep™ Human CD19 Positive Selection Kit Immunomagnetic positive selection of human CD19+ cells (e.g. B cells) 18054 For labeling up to 1 x 10e9 cells 605.00 USD      
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RoboSep™ Human CD19 Positive Selection Kit with Filter Tips EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18054RF For labeling up to 1 x 10e9 cells 641.00 USD      
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Product Type: Cell isolation products
Recommended For:
The isolation of human CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection
Components:
• EasySep™ Human CD19 Positive Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles ,1.0 mL
Equipment Required:
Area of Interest: Chimerism, HIV, HLA, Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: B cells
Popular Product Line: EasySep™, RoboSep™
Selection Method: Positive
Species: Human
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS

Protocols and Manuals

Current documents
Product NameLot# ?Document TypeDownload
EasySep™ Human CD19 Positive Selection Kit All Product Information Sheet
RoboSep™ Human CD19 Positive Selection Kit with Filter Tips All Product Information Sheet
 

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
 

This product has been used in:

  1. Lenka L Allan et al. CD1d and CD1c expression in human B cells is regulated by activation and retinoic acid receptor signaling.J Immunol 186 (9) 5261-5272 (May 1, 2011)
  2. Daniel O Griffin et al. Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+ CD27+ CD43+ CD70-.J Exp Med 208 (1) 67-80 (January 17, 2011)
  3. Leslie J Marshall et al. Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity.J Gen Virol 91 (Pt 12) 3042-3052 (December 2010)
  4. Ikbal Fathallah et al. EBV latent membrane protein 1 is a negative regulator of TLR9.J Immunol 185 (11) 6439-6447 (December 1, 2010)
  5. Mark D Hicar et al. Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV.J Acquir Immune Defic Syndr 54 (3) 223-235 (July 1, 2010)
  6. Nicholas J Megjugorac et al. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.Blood 115 (21) 4185-4190 (May 27, 2010)
  7. Agnes Doreau et al. Interleukin 17 acts in synergy with B cell-activating factor to influence B cell biology and the pathophysiology of systemic lupus erythematosusNat Immunol 10 (7) 778-785 (July 2009)
  8. Nicholas Watkins et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells.Blood 113 (19) e1-9 (May 7, 2009)
  9. Crescent R Isham et al. Chaetocin: a promising new antimyeloma agent with in vitro and in vivo activity mediated via imposition of oxidative stress.Blood 109 (6) 2579-2588 (March 15, 2007)
  10. Kfir Oved et al. A novel postpriming regulatory check point of effector/memory T cells dictated through antigen density threshold-dependent anergy.J Immunol 178 (4) 2307-2317 (February 15, 2007)
  11. Jian Li et al. Human antibodies for immunotherapy development generated via a human B cell hybridoma technology.Proc Natl Acad Sci U S A 103 (10) 3557-3562 (March 7, 2006)

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FACS Histogram Results with EasySep™ CD19+ Positive Selection Kit


FACS Histogram Results with EasySep™ CD19+ Positive Selection Kit
Starting with fresh peripheral blood mononuclear cells, the CD19+ cell content of the enriched fraction typically ranges from 97% - 99%.


Typical EasySep™ Procedure for Human Cells


Typical EasySep™ Procedure for Human Cells



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