Have questions? 1.800.667.0322  | Email Us

EasySep™ Human CD19 Positive Selection Kit

Immunomagnetic Positive Selection Kit

  • EasySep™ Box
  • Box top label for Human CD19 Positive Selection Kit For labeling 10e9 cells
EasySep™ Box
Product Catalog # Size Price
EasySep™ Human CD19 Positive Selection Kit
Immunomagnetic positive selection of human CD19+ cells (e.g. B cells)
18054 For labeling up to 1 x 10e9 cells 620.00 USD
RoboSep™ Human CD19 Positive Selection Kit with Filter Tips
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
18054RF For labeling up to 1 x 10e9 cells 656.00 USD
 

Related Products

Order Offline:

Call Us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email Us:
 
Fax Us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Quick Order

  • EasySep™ Human CD19 Positive Selection Kit (18054)
    For labeling up to 1 x 10e9 cells
    620.00 USD

  • RoboSep™ Human CD19 Positive Selection Kit with Filter Tips (18054RF)
    For labeling up to 1 x 10e9 cells
    656.00 USD

Description


The EasySep™ Human CD19 Positive Selection Kit is designed to isolate CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is expressed on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.
For even faster cell isolations, we recommend the new EasySep™ Human CD19 Positive Selection Kit II (17854) which isolates cells in as little as 18 minutes.
Product Type: Cell isolation products
Recommended For:
The isolation of human CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection
Components:
• EasySep™ Human CD19 Positive Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles ,1.0 mL
Equipment Required:
Area of Interest: Chimerism, HIV, HLA, Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: B cells
Popular Product Line: EasySep™, RoboSep™
Selection Method: Positive
Species: Human
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Technical Resources


Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
EasySep™ Human CD19 Positive Selection Kit All Product Information Sheet
RoboSep™ Human CD19 Positive Selection Kit with Filter Tips All Product Information Sheet

MSDS

Educational Resources

Videos & Webinars

  • EasySep™: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
  • The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
  • EasySep™: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
  • The EasySep™ MultiStand: Four Samples, Four Separations, One Pour-off
  • RoboSep™ Product Tour
  • How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
  • RoboSep™ Sneak Preview
  • Cell Separation Solutions for HLA and Chimerism Analysis
  • Brochures

  • Cell Separation Products for HLA Analysis
  • RoboSep™: Solutions for HIV Research
  • Cell Separation Products for Autoimmunity Research
  • Tools For Your Immunology Research
  • Fast and Easy Isolation of B Cells
  • Fully Automated, High Purity Cell Isolation for Chimerism Labs
  • Wallcharts

  • Frequencies of Cell Types in Human Peripheral Blood Wallchart

  • FAQS


    • HOW EASYSEP™ WORKS

      • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
        A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
      • Q. HOW DOES THE SEPARATION WORK?
        A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
      • Q. WHICH COLUMNS DO I USE?
        A. The EasySep™ procedure is column-free. That's right - no columns!
      • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
        A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
      • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
        A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
      • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
        A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

      EASYSEP™ MAGNETIC PARTICLES

      • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
        A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
      • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
        A. No, but due to the small size of these particles, they will not interfere with downstream applications.
      • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
        A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

        If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

      MODIFYING THE EASYSEP™ PROTOCOL

      • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
        A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
      • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
        A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
     

    Data


    FACS Histogram Results with EasySep™ CD19+ Positive Selection Kit


    FACS Histogram Results with EasySep™ CD19+ Positive Selection Kit
    Starting with fresh peripheral blood mononuclear cells, the CD19+ cell content of the enriched fraction typically ranges from 97% - 99%.


    Typical EasySep™ Procedure for Human Cells


    Typical EasySep™ Procedure for Human Cells



    Related Products


    Other Reagents and Materials


    Product Name

    Description

    Catalog #

    Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells 07801

    Antibodies


    Product Name

    Description

    Catalog #

    Anti-Human CD19 Antibody, Clone HIB19 Mouse Monoclonal Antibody Against Human, Chimpanzee CD19 60005
    Anti-Human CD20 Antibody, Clone 2H7 Mouse Monoclonal Antibody Against Human, Rhesus, Cynomolgus CD20 60008

    Equipment and Accessories


    Product Name

    Description

    Catalog #

    EasySep™ Magnet Immunomagnetic Column-Free Magnet 18000
    "The Big Easy" EasySep™ Magnet Immunomagnetic Column-Free Magnet 18001
    RoboSep™-S RoboSep™-S - The Fully Automated Cell Separator 21000

    Publications


    This Product Has Been Used In:


    Recently Viewed Items