EasySep® Human CD14 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human CD14 Positive Selection Kit For labeling 10e9 cells
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The EasySep® Human CD14 Positive Selection Kit is designed to isolate CD14+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and macrophages and weakly on granulocytes. It is also expressed on most tissue macrophages.
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD14 Positive Selection Kit Immunomagnetic positive selection of human CD14+ cells (e.g. monocytes) 18058 For labeling up to 1 x 10e9 cells 592.00 USD      
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RoboSep® Human CD14 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18058RF For labeling up to 1 x 10e9 cells 625.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD14+ from fresh or previously frozen peripheral blood mononuclear cells by positive selection
Components:
• EasySep® Human CD14 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Chimerism, HIV, HLA, Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: Monocytes
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Pawandeep Dhami et al. Complex exon-intron marking by histone modifications is not determined solely by nucleosome distribution.PLoS One 5 (8) e12339 (2010)
  2. Elisa Bianchi et al. c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression.Blood 116 (22) e99-110 (November 25, 2010)
  3. Anastasia Lambrianides et al. Effects of polyclonal IgG derived from patients with different clinical types of the antiphospholipid syndrome on monocyte signaling pathways.J Immunol 184 (12) 6622-6628 (June 15, 2010)
  4. Nicholas J Megjugorac et al. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.Blood 115 (21) 4185-4190 (May 27, 2010)
  5. Emer P Reeves et al. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.J Immunol 184 (3) 1642-1652 (February 1, 2010)
  6. Benjamin J Capoccia et al. Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity.Blood 113 (21) 5340-5351 (May 21, 2009)
  7. Nicholas Watkins et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells.Blood 113 (19) e1-9 (May 7, 2009)
  8. Alexandra Lambert et al. The C-type lectin surface receptor DCIR acts as a new attachment factor for HIV-1 in dendritic cells and contributes to trans- and cis-infection pathwaysBlood 112 (4) (June 9, 2008)
  9. Caroline Gilbert et al. HIV-1 replication in dendritic cell/T-cell co-cultures is increased upon incorporation of host LFA-1 due to a higher virus productive infection in immature dendritic cellsJ. Virol. JVI.02810-06 (May 9, 2007)
  10. Wayne G Shreffler et al. The major glycoprotein allergen from Arachis hypogaea, Ara h 1, is a ligand of dendritic cell-specific ICAM-grabbing nonintegrin and acts as a Th2 adjuvant in vitro.J Immunol 177 (6) 3677-3685 (September 15, 2006)
  11. Simon L Newman et al. Human macrophages do not require phagosome acidification to mediate fungistatic/fungicidal activity against Histoplasma capsulatum.J Immunol 176 (3) 1806-1813 (February 1, 2006)
  12. Angelique Bellemare-Pelletier et al. HLA-DO transduced in human monocyte-derived dendritic cells modulates MHC class II antigen processingJ Leukoc Biol 78 (1) 95-105 (July 1, 2005)
  13. Cyrile A Curat et al. From blood monocytes to adipose tissue-resident macrophages: induction of diapedesis by human mature adipocytes.Diabetes 53 (5) 1285-1292 (May 2004)
  14. Wei Chen et al. Thrombopoietin cooperates with FLT3-ligand in the generation of plasmacytoid dendritic cell precursors from human hematopoietic progenitors.Blood 103 (7) 2547-2553 (April 1, 2004)

Product Name

Description

Catalog #
CD14 Antibody, Clone MoP9, PE-Conjugated Mouse Monoclonal Antibody to Human CD14 - PE Conjugated 10506
CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results Using EasySep® Human CD14 Positive Selection Kit


FACS Histogram Results Using EasySep® Human CD14 Positive Selection Kit
Starting with fresh peripheral blood mononuclear cells, the CD14+ cell content of the selected fraction typically ranges from 97.8% - 99.7%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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