EasySep® Human CD138 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human CD138 Positive Selection Kit For labeling up to 2 x 10e9 cells
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The EasySep® Human CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1)cells from fresh or previously frozen bone marrow or peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. This cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).
Product Name Description Catalog # Size Price Quantity
EasySep® Human CD138 Positive Selection Kit Immunomagnetic positive selection of human CD138+ cells (e.g. normal or malignant plasma cells for multiple myeloma research) 18357 For labeling up to 2 x 10e9 cells 592.00 USD      
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RoboSep® Human CD138 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18357RF For labeling up to 2 x 10e9 cells 625.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD138+ cells from fresh or previously frozen bone marrow or peripheral blood mononuclear cells by positive selection
Components:
• EasySep® Human CD138 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Cancer, Hematologic malignancies, Immunology
Cell Source: Bone marrow, PBMC
Cell Type: B cells, Plasma cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. James W Clendening et al. Exploiting the mevalonate pathway to distinguish statin-sensitive multiple myeloma.Blood 115 (23) 4787-4797 (June 10, 2010)
  2. Nellie Dumont et al. Increased secretion of hyperimmune antibodies following lipopolysaccharide stimulation of CD40-activated human B cells in vitro (February 18, 2010)
  3. Ulrike Bacher et al. Correlation of Cytomorphology, Immunophenotyping, and Interphase Fluorescence in Situ Hybridization (FISH) in 381 Patients with MGUS and 310 Patients with Multiple Myeloma.Blood 114 2812 (November 2009)
  4. Borte S et al. Interleukin-21 restores immunoglobulin production ex vivo in patients with common variable immunodeficiency and selective IgA deficiencyBlood 114 (19) 4089-4098 (November 5, 2009)
  5. Bonnie Arendt et al. Biologic and genetic characterization of the novel amyloidogenic lambda light chain???secreting human cell lines, ALMC-1 and ALMC-2Blood 112 1931-1941 (September 2008)
  6. Bonnie Arendt et al. Biologic and genetic characterization of the novel amyloidogenic lambda light chain-secreting human cell lines, ALMC-1 and ALMC-2Blood 112 (5) (September 1, 2008)
  7. Jaime Darce et al. Regulated Expression of BAFF-Binding Receptors during Human B Cell DifferentiationJ. Immunol. 179 7276-7286 (December 2007)
  8. Daniels Tr et al. Conjugation of an anti transferrin receptor IgG3-avidin fusion protein with biotinylated saporin results in significant enhancement of its cytotoxicity against malignant hematopoietic cellsMol Cancer Ther 6 (11) 2995-3008 (November 2007)
  9. M Kline et al. ABT-737, an inhibitor of Bcl-2 family proteins, is a potent inducer of apoptosis in multiple myeloma cellsLeukemia 21 (7) 1549-1560 (April 26, 2007)
  10. Crescent Isham et al. http://abstracts.hematologylibrary.org/cgi/content/abstract/114/22/2812?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&andorexacttitle=and&andorexacttitleabs=and&fulltext=%22CD138%22+AND+%22RoboSep%22&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&resourcetype=Blood 109 2579-2588 (March 2007)
  11. Patrick P Ng et al. Molecular events contributing to cell death in malignant human hematopoietic cells elicited by an IgG3-avidin fusion protein targeting the transferrin receptor.Blood 108 (8) 2745-2754 (October 15, 2006)
  12. M Timm et al. Thymoglobulin targets multiple plasma cell antigens and has in vitro and in vivo activity in multiple myelomaLeukemia 20 (10) 1863-1869 (August 17, 2006)

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results With EasySep® Human CD138 Positive Selection Kit


FACS Histogram Results With EasySep® Human CD138 Positive Selection Kit
Starting with fresh peripheral blood mononuclear cells, the CD138+/Syndecan-1 cell content of the selected fraction typically ranges from 85% - 95%. Please note that purity is highly dependent on the starting sample.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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