EasySep® Human Buffy Coat CD14 Positive Selection Kit

? 1.800.667.0322  • > Contact Us

Immunomagnetic Positive Selection Kit

Email  Print


Free from STEMCELL

  • EasySep® box
  • Box top label for Human Buffy Coat CD14+ Positive Selection Kit For labeling 30 mL buffy coat
EasySep® box
The EasySep® Human Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Buffy Coat CD14 Positive Selection Kit Immunomagnetic positive selection of human CD14+ cells (e.g. monocytes) from buffy coat 18088 For labeling 30 mL of buffy coat 507.00 USD      
 Save Item
RoboSep® Human Buffy Coat CD14 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18088RF For labeling 30 mL of buffy coat 568.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD14+ cells from buffy coat by positive selection
Components:
• EasySep® Human Buffy Coat CD14 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep® Magnetic Particles, 3 x 1.0 mL
• EasySep® RBC Lysis Buffer 10X Concentrate, 10 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Chimerism, HIV, HLA, Immunology
Cell Isolation Product Type: Reagents
Cell Source: Buffy coat, Whole blood
Cell Type: Monocytes
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800
CD14 Antibody, Clone MoP9, PE-Conjugated Mouse Monoclonal Antibody to Human CD14 - PE Conjugated 10506
CD16 Antibody, Clone NKP15, FITC-Conjugated Mouse Monoclonal Antibody to Human CD16 - FITC Conjugated 10408
RoboSep® Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer 20110
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406
StemSpan® SFEM Serum-Free Medium for Expansion of Hematopoietic Cells 09600
StemSpan® SFEM 09650
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Human Pan-DC Pre-Enrichment Kit Immunomagnetic Negative Selection Kit 19251
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000

FACS Histogram Results Using EasySep® Human Buffy Coat CD14 Positive Selection Kit


FACS Histogram Results Using EasySep® Human Buffy Coat CD14 Positive Selection Kit
Starting with buffy coat, the CD14+ cell content of the selected fraction typically ranges from 96.5% - 99.6%. *Red blood cells were removed by lysis prior to flow cytometry.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



Recently Viewed Items