EasySep® Human Biotin Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Human Biotin Selection Kit For labeling 10e9 cells
EasySep® box
The EasySep® Human Biotin Positive Selection Kit is designed to isolate cells that are labeled with biotinylated antibodies. The cocktail contains a combination of antibodies that is directed against biotin and dextran. Desired cells are targeted with Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Biotin Positive Selection Kit Immunomagnetic positive selection of any human cells labeled with biotinylated antibodies 18553 For labeling up to 1 x 10e9 cells 333.00 USD      
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RoboSep® Human Biotin Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18553RF For labeling up to 1 x 10e9 cells 366.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human cells labeled with biotinylated antibodies by positive selection
Components:
• EasySep® Biotin Selection Cocktail, 1.0 mL
• Anti-Human CD32 FcR Blocker, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
• RoboSep® Vial for Primary Conjugated Antibody (not required for manual use), 1 vial
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: Other
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Kei Tomihara et al. Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.J Immunol 184 (11) 6151-6160 (June 1, 2010)
  2. Heike Wulff et al. K+ channel expression during B cell differentiation: implications for immunomodulation and autoimmunity.J Immunol 173 (2) 776-786 (July 15, 2004)

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Human PE Positive Selection Kit Immunomagnetic Positive Selection Kit 18551
EasySep® Human FITC Positive Selection Kit Immunomagnetic Positive Selection Kit 18552
EasySep® Human APC Positive Selection Kit Immunomagnetic Positive Selection Kit 18451

FACS Histogram Results With EasySep® Biotin Selection Kit for Human Cells Example: Human CD3+ Cells


FACS Histogram Results With EasySep® Biotin Selection Kit for Human Cells Example: Human CD3+ Cells



Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



Schematic drawing of EasySep® Magnetic Labeling of Any Cell Type


Schematic drawing of EasySep® Magnetic Labeling of Any Cell Type



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