EasySep® Human B Cell Enrichment Kit

Recommended for:	The isolation of human B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection
Components:	• EasySep® Human B Cell Enrichment Cocktail, 1.0 mL • EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• EasySep® Magnet (Catalog #18000), or • “The Big Easy” EasySep® Magnet (Catalog #18001), or • "Easy50" EasySep® Magnet (Catalog #18002), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Cell Type:	B cells
Popular Product Line:	EasySep, RoboSep
Area of Interest:	Immunology, HIV
Species:	Human
Cell Source:	PBMC
Selection Method:	Negative
Cell Isolation Product Type:	Reagents

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 19054, Lot# 10C33933 or less:28744-PIS_3_0_2.pdf
•  PRODUCT INFORMATION SHEET - 19054, Lot# 10E35004 or greater:28744-PIS_4_3_0.pdf
•  PRODUCT INFORMATION SHEET - 19054, Lot# 10E35004 or greater:28744-EasyPlate-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19054, Lot# 10E35004 or greater:28744-Easy50-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19054RF, Lot# 10C33933 or less:28744-PIS_3_0_2.pdf
•  PRODUCT INFORMATION SHEET - 19054RF, Lot# 10E35004 or greater:28744-PIS_4_3_0.pdf

Educational resources:

•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: RoboSep®: Solutions for HIV Research
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  WALLCHART: Antigen Processing and Presentation Wallchart
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  BROCHURE: Fast and Easy Isolation of B Cells
•  WALLCHART: Frequencies of Cell Types in Human Peripheral Blood Wallchart

MSDS:

•  19250_19250H-MSDS.pdf
•  19014C.2_19054C.2-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE CELLS FROM WHOLE BLOOD?
  
  A. Yes.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Xiaosheng Wu et al. Selective induction of DNA repair pathways in human B cells activated by CD4+ T cells.PLoS One 5 (12) e15549 (2010)
2. Stephen A Mihalcik et al. The structure of the TNFRSF13C promoter enables differential expression of BAFF-R during B cell ontogeny and terminal differentiation.J Immunol 185 (2) 1045-1054 (July 15, 2010)
3. Lenka L Allan et al. Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells.Blood 114 (12) 2411-2416 (September 17, 2009)
4. K B Douglas et al. Complement receptor 2 polymorphisms associated with systemic lupus erythematosus modulate alternative splicing.Genes Immun 10 (5) 457-469 (July 2009)
5. Xiaosheng Wu et al. Alternative splicing regulates activation-induced cytidine deaminase (AID): implications for suppression of AID mutagenic activity in normal and malignant B cellsBlood 112 (12) (December 1, 2008)
6. Genevieve Martin et al. Human immunodeficiency virus type 1-associated CD40 ligand transactivates B lymphocytes and promotes infection of CD4+ T cells.J. Virol. 81 (11) 5872-5881 (June 1, 2007)
7. Jaime R. Darce et al. Divergent Effects of BAFF on Human Memory B Cell Differentiation into Ig-Secreting CellsJ Immunol 178 (9) 5612-5622 (May 1, 2007)
8. Kumudha Balakrishnan et al. Forodesine, an inhibitor of purine nucleoside phosphorylase, induces apoptosis in chronic lymphocytic leukemia cells.Blood 108 (7) 2392-2398 (October 1, 2006)

Product Name	Description	Catalog #
CD19 Antibody, Clone 4G7, PE-Conjugated	Mouse Monoclonal Antibody to Human CD19 - PE Conjugated	10509
CD22 Antibody, Clone SHCL-1, FITC-Conjugated	Mouse Monoclonal Antibody to Human CD22 - FITC Conjugated	10411
CD20 Antibody, Clone L27, PE-Conjugated	Mouse Monoclonal Antibody to Human CD20 - PE Conjugated	10510
Ficoll-Paque™ PLUS	Density Gradient Medium for the Isolation of Mononuclear Cells	07907
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000

FACS Histogram Results With EasySep® Human B Cell Enrichment Kit

Starting with frozen mononuclear cells, the CD19+ cell content of the enriched fraction typically ranges from 95% - 99%.

Typical EasySep® Procedure for Human Cells