EasySep® HLA Whole Blood CD33 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • EasySep® box
  • Box top label for HLA WB CD33 Positive Selection Kit For labeling 60 mL of buffy coat or whole blood
EasySep® box
The EasySep® HLA CD33 Positive Selection Kit is designed to isolate CD33+ cells from whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. This cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.
Product Name Description Catalog # Size Price Quantity
EasySep® HLA Whole Blood CD33 Positive Selection Kit Column-free immunomagnetic positive selection of human CD33+ cells (e.g. myeloid cells) from whole blood for HLA analysis 18287HLA For labeling 60 mL of whole blood 492.00 USD      
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RoboSep® HLA Whole Blood CD33 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18287HLARF For labeling 60 mL of whole blood 551.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD33+ cells from whole blood by positive selection for HLA analysis
Components:
• EasySep® HLA Whole Blood CD33 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep® Magnetic Particles, 3 x 1.0 mL
• EasySep® RBC Lysis Buffer 10X Concentrate, 10 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Chimerism, HLA
Cell Isolation Product Type: Reagents
Cell Source: Whole blood
Cell Type: Myeloid cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

Product Name

Description

Catalog #
RoboSep® Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer 20110
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000

FACS Histogram Results with EasySep® HLA Buffy Coat/Whole Blood CD33Positive Selection Kit


FACS Histogram Results with EasySep® HLA Buffy Coat/Whole Blood CD33Positive Selection Kit
Starting with fresh whole blood, the CD33+ cell content of the enriched fraction (asassessed by antibodies against CD14 and CD66b) typically ranges from 82.3% - 97.4%.


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