EasySep® HLA Buffy Coat CD56 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

EasySep® box

The EasySep® HLA Buffy Coat CD56 Positive Selection Kit is designed to isolate CD56+ cells from buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.

Product Name	Description	Catalog #	Size	Price	Quantity
EasySep® HLA Buffy Coat CD56 Positive Selection Kit	Column-free immunomagnetic positive selection of human CD56+ cells (e.g. NK cells) from buffy coat for HLA analysis	18085HLA	For labeling 30 mL of buffy coat	492.00 USD		Save Item
RoboSep® HLA Buffy Coat CD56 Positive Selection Kit with Filter Tips	EasySep® kit with RoboSep® buffer and RoboSep® filter tips	18085HLARF	For labeling 30 mL of buffy coat	551.00 USD		Save Item

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Recommended for:	The isolation of human CD56+ cells from buffy coat by positive selection for HLA analysis
Components:	• EasySep® HLA Buffy Coat CD56 Positive Selection Cocktail, 3 x 1.0 mL • EasySep® Magnetic Particles, 3 x 1.0 mL • EasySep® RBC Lysis Buffer 10X Concentrate, 10 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• “The Big Easy” EasySep® Magnet (Catalog #18001), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Area of Interest:	Chimerism, HLA
Cell Isolation Product Type:	Reagents
Cell Source:	Buffy coat, Whole blood
Cell Type:	NK cells
Popular Product Line:	EasySep, RoboSep
Selection Method:	Positive
Species:	Human

Procedures and instruction manuals:

PRODUCT INFORMATION SHEET - 18085HLA, Lot# All:29097-PIS_1_0_0.pdf
PRODUCT INFORMATION SHEET - 18085HLARF, Lot# All:29097-PIS_1_0_0.pdf

Educational resources:

MSDS:

FAQS:

HOW EASYSEP™ WORKS
- Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
- Q. HOW DOES THE SEPARATION WORK?
  A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
- Q. WHICH COLUMNS DO I USE?
  A. The EasySep™ procedure is column-free. That's right - no columns!
- Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
- Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
  A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
- Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
  A. Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
EASYSEP™ MAGNETIC PARTICLES
- Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
  A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
- Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
  A. No, but due to the small size of these particles, they will not interfere with downstream applications.
- Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
  A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.
  
  If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
MODIFYING THE EASYSEP™ PROTOCOL
- Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
- Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
  A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Product Name	Description	Catalog #
Ammonium Chloride Solution	Reagent for the Lysis of Red Blood Cells	07800
CD56 Antibody, Clone NCAM16.2, PE-Conjugated	Mouse Monoclonal Antibody to Human - CD56 PE Conjugated	10526
CD3 Antibody, Clone SK7, PE-Conjugated	Mouse Monoclonal Antibody to Human CD3 - PE Conjugated	10502
CD3 Antibody, Clone SK7, FITC-Conjugated	Mouse Monoclonal Antibody to CD3 - FITC Conjugated	10402
RoboSep® Red Blood Cell Lysis Buffer	Red Blood Cell Lysis Buffer	20110
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000