Easy 50 EasySep® Magnet

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Immunomagnetic Column-Free Magnet

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"Easy 50" EasySep® Magnet

The "Easy 50" EasySep® Magnet is designed for cell separation procedures using selected EasySep® reagents when processing a large number of cells (up to 4 x 10e9 cells). The "Easy 50" Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep® Magnetic D Particles without the use of columns. The "Easy 50" EasySep® Magnet is designed to hold a standard 50 mL conical tube.

Product Name	Description	Catalog #	Size	Price	Quantity
Easy 50 EasySep® Magnet	Immunomagnetic column-free magnet	18002	For isolating up to 4 x 10e9 cells	1246.00 USD		Save Item

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Recommended for:	Processing up to 4 x 10e9 cells (HetaSep™-treated whole blood or mouse splenocytes) or 2 x 10e9 cells (PBMC)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type:	Cell isolation products
Popular Product Line:	EasySep
Cell Isolation Product Type:	Equipment

Procedures and instruction manuals:

PRODUCT INFORMATION SHEET - 18002, Lot# All:29142-PIS_2_0_0.pdf

Educational resources:

FAQS:

Q. HOW DOES THE SEPARATION WORK?

A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
Q. WHICH COLUMNS DO I USE?

A. None, columns are not used in the EasySep^® procedure.
Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

A. Yes, but this may affect separation performance.
Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

A. No, but they are so tiny, they do not interfere with downstream applications.
Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?

A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

A. Yes, we have products for both.
Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

A. Each Product Information Sheet has detailed staining information.
Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

A. No, we have found that cells are not functionally altered.
Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?

A. Yes, click here for a link to RoboSep^®.