Anti-Mouse CD44 Antibody, Clone IM7

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Rat Monoclonal Antibody Against Human, Mouse, Rhesus CD44

  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 100μg
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 25 µg
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, Biotin 500μg
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, Biotin 50 µg
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, PE 100 tests
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7, PE 25 tests
  • Vial label for Anti-Mouse CD44 Antibody, Clone IM7 500μg
Vial label for Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 100μg
The IM7 antibody reacts with CD44 (Ly-24), an ~80 - 95 kDa type 1 transmembrane glycoprotein involved in cell-cell and cell-matrix interactions. CD44 is expressed on the surface of many cells, including leukocytes, hepatocytes, endothelial, epithelial, and mesenchymal cells. Expression levels increase upon activation of T and B cells, and memory cells exhibit a CD44[High] phenotype. CD44 binds many ligands, including hyaluronic acid, collagen, fibronectin, growth factors and metalloproteinases, thus modulating processes such as lymphocyte activation, recirculation and homing, leukocyte rolling and aggregation, hematopoiesis, and tumor metastasis. Numerous disorders are associated with altered expression or dysfunction of CD44. Many CD44 isoforms have been identified, with alternative splicing, differential N- and O- glycosylation, and sulfation mediating the functional role(s) played by the protein in a specific cell. The IM7 monoclonal antibody reacts with an extracellular epitope found on all isoforms of CD44 and both murine allotypes.
 
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Enrichment Kit (Catalog #19752).
Product Name Description Catalog # Size Price Quantity
Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, Alexa Fluor® 488-conjugated 60068AD 100 µg 239.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, Alexa Fluor® 488-conjugated 60068AD.1 25 µg 105.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7, Biotin Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, biotin-conjugated 60068BT 500 µg 269.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7, Biotin Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, biotin-conjugated 60068BT.1 50 µg 69.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7, PE Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, PE-conjugated 60068PE 100 tests 155.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7, PE Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, PE-conjugated 60068PE.1 25 tests 75.00 USD      
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Anti-Mouse CD44 Antibody, Clone IM7 Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44, unconjugated (Tissue Non-Specific Alkaline Phosphatase) 60068 500 µg 239.00 USD      
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Species Reactivity: Baboon, Cat, Chimpanzee, Cow, Dog, Horse, Human, Macaque, Monkey, Mouse, Pig
Host Species: Rat
Application: For the appropriate antibody/conjugate:, Cell isolation, CyTOF, ELISA, Flow cytometry, Functional assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Product Type: Antibodies
Antibody Type: Primary antibodies
Area of Interest: Immunology
Cell Type: B cells, Dendritic cells, Granulocytes & subsets, Hematopoietic stem & progenitor cells, Lymphocytes, Monocytes, Myeloid cells, NK cells, Red blood cells, Regulatory T cells, T cells
Conjugation: Alexa Fluor, Biotin, PE, Unconjugated
Legal Statement: Alexa Fluor® is a registered trademark of Life Technologies Corporation. This product is licensed for internal research use only and its sale is expressly conditioned on the buyer not using it for manufacturing, performing a service, or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Protocols and Manuals

Current documents
Product NameLot# ?Document TypeDownload
Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 All Product Information Sheet
Anti-Mouse CD44 Antibody, Clone IM7, Biotin All Product Information Sheet
Anti-Mouse CD44 Antibody, Clone IM7, PE All Product Information Sheet
Anti-Mouse CD44 Antibody, Clone IM7 All Product Information Sheet
 

Educational resources:

MSDS:

 

Product Name

Description

Catalog #

EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit NEW: 15-Minute Cell Isolation Kit using Immunomagnetic Negative Selection 19765

Data for Unconjugated


60068
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, followed by goat antimouse IgG, FITC (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (open histogram). Data was gated on the total lymphocyte population.


Data for Alexa Fluor® 488-conjugated


60068AD
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).


Data for Biotin-conjugated


60068BT
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibody followed by SAV APC (solid line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibodyfollowed by SAV APC (open histogram).


Data for PE-conjugated


60068PE
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).


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