10% Bovine Serum Albumin in Iscove's MDM

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10% Bovine Serum Albumin (BSA) in Iscove's MDM

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  • Bovine Serum Albumin 100 mL
  • Label for Bovine Serum Albumin 100 mL
Bovine Serum Albumin 100 mL
This product has been developed for use in applications where a culture medium of defined composition is required. It contains pre-screened batches of bovine serum albumin (BSA) that have been selected to support the optimal growth of human hematopoietic progenitor cells in serum-free media formulations. It is also suitable for the culture of mouse hematopoietic progenitors in serum-free conditions.
Product Name Description Catalog # Size Price Quantity
10% Bovine Serum Albumin in Iscove's MDM 10% bovine serum albumin in Iscove's MDM 09300 100 mL 794.00 USD      
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Recommended for:
Use with MethoCult® H4100 (human cells) or MethoCult® M3134 (mouse cells) for hematopoietic progenitor colony-forming cell (CFC) assays (at a final concentration of 1%)
Accessory Products:
• MethoCult® H4100 (Catalog #04100)
• MethoCult® M3134 (Catalog #03134)
• Iscove's Modified Dulbecco's Medium (IMDM) with 2% Fetal Bovine Serum (Catalog #07700)
• Blunt-end Needles (Catalog #28110/28120)
• 3 cc Syringe (Catalog #28230/28240)
• 35 mm Culture Dishes (Catalog #27100/27150
• 60 mm Gridded Scoring Dishes (Catalog #27500)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Bovine Serum Albumin (10%)
• Iscove's MDM (buffered with NaHCO3)
Other cell culture media, reagents & supplies: Sera & serum substitutes
Cell Type: Hematopoietic stem & progenitor cells, Hematopoietic cells
Area of Interest: Hematopoietic stem cell research
Application: Maintenance
Source: Bovine

Procedures and instruction manuals:

Educational resources:

MSDS:

Background References:

  1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
  2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
  3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
  4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
  5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
  6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
  7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
  8. A L Petzer et al. Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.J Exp Med 183 (6) 2551-2558 (June 1, 1996)
  9. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
  10. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name

Description

Catalog #
BIT 9500 Serum Substitute Serum Substitute for Culturing Human and Mouse Hematopoietic Cells 09500
10% Human Serum Albumin in Iscove's MDM 10% Human Serum Albumin (HSA) in Iscove's MDM 09350
HIT Serum Substitute Serum Substitute for Culturing Human Hematopoietic Cells 09550
MethoCult® M3134 Base Methylcellulose Medium for Mouse Cells 03134
MethoCult® H4100 Base Methylcellulose Medium for Human Cells 04100
Iscove's MDM with 2% FBS Medium for Preparing and Washing Samples for Colony-Forming Cell (CFC) Assays 07700
35 mm Culture Dishes 35 mm Culture Dishes 27100
60 mm Gridded Scoring Dishes Dishes for Reproducible and Accurate Scoring of Colonies 27500
Blunt-End Needles, 16 Gauge 16 Gauge Blunt-end Needles 28110
3 cc Syringes 3 cc Syringes 28230

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