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2009 | 2008 | 2007 | 2006 | 2005 | 2004 2009-01-23
Now Available! NeuroCult®-ACF Proliferation Medium (Catalog #05761) for the animal component-free culture of human NSCs
STEMCELL Technologies is delighted to announce release of NeuroCult®-ACF Proliferation Medium (Catalog #05761) for the animal component-free, serum-free culture of human neural stem and progenitor cells.
Given the interest in using neural stem cells for applications in regenerative medicine, there is a need for animal component-free culture systems. NeuroCult®-ACF Proliferation Medium is designed to support prolonged and reproducible expansion of human NSCs in culture. Neurospheres can be efficiently generated and expanded in NeuroCult®-ACF Proliferation Medium and NSCs maintain multi-lineage potential in long-term cultures (Figures 1 and 2, respectively).
Figure 1: Neurospheres derived from human fetal CNS tissue can be efficiently generated for multiple passages in NeuroCult®-ACF Proliferation Medium.
Total cell expansion obtained with NeuroCult®-ACF Proliferation Medium is comparable to that obtained with NeuroCult® NS-A Proliferation Medium (Human).
Figure 2: NSCs cultured in NeuroCult®-ACF Proliferation Medium maintain multi-lineage potential in long-term cultures.
Cells obtained from passage 10 neurospheres cultured in NeuroCult®-ACF Proliferation Medium were differentiated under animal component-free differentiation conditions. Immunofluorescent staining was performed to identify the differentiated cell types.
A. Neurons (red) were detected with an anti-ß-Tubulin III antibody and astrocytes (green) with an anti-GFAP antibody.
B. Oligodendrocytes (green) were detected with an anti-O4 Oligodendrocyte Marker antibody and cell nuclei (blue) were identified using the DNA dye DAPI.
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