TeSR™2

Recommended for:	Maintenance culture of undifferentiated hESCs or hiPSCs
Components:	• TeSR™2 Basal Medium, 400 mL (Catalog #05861) • TeSR™2 5X Supplement, 100mL (Catalog #05862) • TeSR™2 250X Supplement, 2mL (Catalog #05863)
Accessory Products:	• mTeSR®1 (Catalog #05850) • AggreWell™400 plates (Catalog #27845/27945) • Oct 3/4 Antibody, Clone 40 (Catalog #01550 / 01551) • SSEA-1 Antibody, Clone MC-480 (Catalog #01552) • SSEA-3 Antibody, Clone MC-631 (Catalog #01553) • SSEA-4 Antibody, Clone 813-70 (Catalog #01554) • TRA-1-60 Antibody, Clone TRA-1-60 (Catalog #01555) • TRA-1-81 Antibody, Clone TRA-1-81 (Catalog #01556) • TRA-2-49 Antibody, Clone TRA-2-49/6E (Catalog #01557) • TRA-2-54 Antibody, Clone TRA-2-54/2J (Catalog #01558) • mFreSR® Defined Cryopreservation Medium (Catalog #5855/05854) • CryoStor™CS10 Defined, Animal Protein-Free Cryopreservation Medium (Catalog #07930) • ACCUTASE™ (Catalog #07920) • Dispase (1 mg/mL) (Catalog #07923)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:	The formulation does not contain any animal proteins.
Equipment Required:	N/A
Legal Statement:	This product was developed under license to intellectual property owned by WiCell™ Research Institute (patent pending). This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers wishing to use the product for purposes other than research use should contact Geron Corporation’s Business Development office at (650) 473-7700 or corpdev@geron.com. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.
Product Type:	Specialized cell culture media
Application:	Maintenance
Area of Interest:	Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type:	Embryonic stem cells & iPS cells
Popular Product Line:	mTeSR1 and TeSR2
Species:	Human

Procedures and instruction manuals:

•  MANUAL: Maintenance of hESCs and hiPSCs in mTeSR®1 and TeSR™2
•  PRODUCT INFORMATION SHEET - 05860, Lot# All:29930-PIS_1_0_1.pdf

Educational resources:

•  WEBINAR: Standardizing hESC and hiPSC Maintenance and Differentiation
•  VIDEO: TeSR™2: A Protein-Free Maintenance Medium for hESCs and hiPSCs
•  BROCHURE: Standardized Solutions for hESC & hiPSC Research
•  BROCHURE: TeSR™2: Animal Protein-Free Maintenance Medium
•  BROCHURE: Purifying CD34+ Cells Differentiated From Human Pluripotent Stem Cells
•  WALLCHART: Pluripotent Stem Cell Biology Wallchart
•  MINI REVIEW: Mini-review: Induced pluripotent stem cells (Catalog #29143)
•  TECH BULLETIN: Transitioning Human Embryonic and Induced Pluripotent Stem Cells from TeSR™2 to mTeSR™1

MSDS:

•  05862-MSDS_Lots_beginning_11.pdf
•  05862-MSDS_Lots_beginning_10.pdf
•  05863-MSDS.pdf
•  05861-MSDS.pdf

This product has been used in:

1. Kazim H Narsinh et al. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells.J Clin Invest (February 7, 2011)
2. Ryan Lister et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells.Nature (February 2, 2011)

Background References:

1. In-Hyun Park et al. Reprogramming of human somatic cells to pluripotency with defined factors.Nature 451 (7175) 141-146 (December 23, 2007)
2. Kazutoshi Takahashi et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors.Cell 131 (5) 861-872 (November 20, 2007)
3. Jonathan S Draper et al. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.J Anat 200 (Pt 3) 249-258 (March 2002)
4. J A Thomson et al. Embryonic stem cell lines derived from human blastocysts.Science 282 (5391) 1145-1147 (November 6, 1998)

Product Name	Description	Catalog #
mTeSR®1	Defined, Feeder-Independent Maintenance Medium for hESCs and hiPSCs	05850
AggreWell™	Simple and Standardized Production of Embryoid Bodies	27845
mFreSR®	Defined, Serum-Free Cryopreservation Medium for hESCs and hiPSCs	05854
CryoStor™CS10	Animal Protein-Free, Defined Cryopreservation Medium	07930
EasySep® hESC-Derived CD34 Positive Selection Kit	Immunomagnetic Positive Selection Kit	18167
Oct-3/4 Antibody, Clone 40	Mouse Monoclonal Antibody to Oct-3/4	01550
Oct-3/4 Antibody, Clone 40		01551
SSEA-3 Antibody, Clone MC-631	Rat Monoclonal Antibody to SSEA-3	01553
SSEA-1 Antibody, Clone MC-480	Mouse Monoclonal Antibody to SSEA-1	01552
SSEA-4 Antibody, Clone 813-70	Mouse Monoclonal Antibody to Anti-SSEA-4	01554
TRA-1-81 Antibody, Clone TRA-1-81	Mouse Monoclonal Antibody to TRA-1-81	01556
Rabbit IgG (H+L) Antibody, FITC-Conjugated	Goat Polyclonal Antibody to Rabbit IgG (H+L) - FITC Conjugated	10212
IgM Antibody, FITC-Conjugated	Goat Polyclonal Antibody to Mouse IgM - FITC Conjugated	10211
IgM Antibody, APC-Conjugated	Goat Polyclonal Antibody to Rat IgM - APC Conjugated	10215
Y-27632		07172
ACCUTASE™	ACCUTASE™ Cell Detachment Solution	07920
Dispase (1 mg/mL)	Dispase 1 mg/mL	07923

Expansion of hESCs cultured in TeSR™2

hESCs cultured in TeSR™2 demonstrate consistent expansion.

Morphology of hiPSCs cultured in TeSR™2

hiPSCs cultured in TeSR™2 grow as colonies with defined edges and high nucleus to cytoplasm ratio. iPS(IMR90)-1 cell line cultured (A) for 8 passages in TeSR™2 and (B) for 10 passages in TeSR™2.

hESCs cultured long-term in TeSR™2 retain normal karyotype

Retention of normal karyotype in (A) H1 and (B) H9 cells following long-term passaging in TeSR™2, 19 and 22 passages respectively.

hESCs cultured in TeSR™2 are pluripotent

H9 cells were cultured for 11 passages in TeSR™2 then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown. resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown.

Human embryonic stem cells cultured in either mTeSR®1 or TeSR™2 express high levels of pluripotent markers and low levels of differentiation markers

Flow cytometric analysis of H1 cells maintained in TeSR™2 for 26 passages.