Hematopoietic stem cells & progenitor cells

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STEMvision™ automated & standardized CFC enumeration

STEMvision for Imaging and Scoring Hematopoietic Colonies

STEMvision™ is an automated instrument and computer system designed specifically for imaging and scoring hematopoietic colonies in the colony-forming cell (CFC) assay using our optimized MethoCult® media and our new meniscus-reducing SmartDishesTM.
STEMvision™ can help you:

Digitally image cultures for permanent record keeping
Automate and standardize CFC scoring to reduce variability
Collect and review data in an easy-to-use format

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Cell isolation

Isolate Hematopoietic Progenitor Cells with EasySep

Hematopoietic progenitor cells can be isolated easily and quickly by either depletion of lineage-specific markers (for human and mouse), or by the positive selection of CD34 (human), or c-kit and Sca-1 (mouse) using STEMCELL’s immunomagnetic, column-free system, EasySep®. EasySep® can be performed manually, or can be automated with RoboSep®, the fully automated cell separator. For the rapid de-bulking of lineage-specific markers from whole blood, STEMCELL also offers a RosetteSep® kit which crosslinks Lin positive cells to red blood cells for depletion.

Isolated cells are highly functional and immediately ready for downstream assays.

Methylcellulose-based colony assay media

Quantitation of Hematopoietic Progenitors with Colony Assays in Methylcellulose

Quantitation of hematopoietic progenitors is commonly done with the use of colony assays, where test cells are plated at low cell density into semi-solid medium containing appropriate growth factors. These assay conditions allow the proliferation and differentiation of progenitor cells into mature cell types, which can then be distinguished morphologically.

Several different semi-solid media have been used for hematopoietic colony assays including methylcellulose, agar and collagen. Methylcellulose is now the standard medium for these assays because it permits improved growth of erythroid colonies over other types of semi-solid support systems (e.g. agar), while allowing optimal growth of granulocyte and macrophage colonies. Committed progenitors for both erythroid and granulocyte/macrophage lineages, as well as multi-potential progenitors, can therefore be assayed simultaneously in the same culture dish.

Collagen-based colony assay media

Quantitation of Hematopoietic Progenitors with Colony Assays in Collagen

Collagen gels have also been shown to support the proliferation of hematopoietic progenitors. They have the advantage that they can be dehydrated and fixed for subsequent cytochemical and immunocytochemical analysis, making them an ideal system for the detection and quantitation of megakaryocytic progenitors or other progenitors that require more detailed analysis than the standard morphological criteria that are commonly used. Furthermore, since collagen gels can be fixed, long-term records can be established.

Long-term culture media

Long-term culture of hematopoietic progenitors is primarily used to quantitate those progenitors capable of initiating and sustaining myelopoiesis for several weeks in vitro. These cells share functional and phenotypic properties with in vivo repopulating cells and are commonly referred to as long-term culture-initiating cells (LTC-IC). MyeloCult^® long-term culture media promote the formation of supportive human or mouse stromal layers and allow the proliferation and differentiation of primitive hematopoietic progenitors.

Sera

Serum for Hematopoietic Cell Growth and Differentiation

Fetal bovine sera (FBS) and horse sera are rigorously screened and selected based on their ability to support hematopoietic cell growth and differentiation. Sera are available for several applications, including myeloid colony-forming cell assays, myeloid long-term cultures, pre-B lymphoid colony-forming cell assays and B-lymphoid long-term cultures.

Serum-free media

Serum-free Cell Culture Media for Hematopoietic Cell Growth and Differentiation

Historically, medium containing fetal bovine serum (FBS) has been used to culture hematopoietic cells. However, the concentrations of inhibitors including transforming growth factor-beta and other constituents vary between batches of sera. In order to minimize these variables, particularly for studies of the effects of specific regulatory or growth factors, there has been increased interest in the use of serum-free media. A variety of serum-free media is available for many applications.

Stem cell detection

ALDEFLUOR – Detect Hematopoietic Stem & Progenitor Cells

The ALDEFLUOR^® and ALDECOUNT^® reagent systems are non-immunological methods to identify human stem/progenitor cells from bone marrow, peripheral blood, apheresis collections and umbilical cord blood on the basis of their aldehyde dehydrogenase (ALDH) activity. High ALDH activity has also been found in other cell types. Only cells with an intact cellular membrane can retain the ALDEFLUOR^®/ALDECOUNT^® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Primary cells

Purified frozen hematopoietic cells are available.

Antibodies

Antibodies are available against human, mouse or rhesus monkey hematopoietic cells

Cytokines

A wide range of cytokines are available for use in human or mouse cell cultures.

Cell cultureware

STEMCELL Technologies provides a complete selection of cell cultureware and equipment to complement our other cell culture products.

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Product Name	Description	Catalog #	Size	Price
10% Bovine Serum Albumin in Iscove's MDM	10% bovine serum albumin in Iscove's MDM	09300	100 mL	794.00 USD
10% Human Serum Albumin in Iscove's MDM	10% human serum albumin in Iscove's MDM	09350	100 mL	576.00 USD
35 mm Culture Dishes	35 mm culture dishes	27150	500 Dishes/Pack	251.00 USD
35 mm Culture Dishes	35 mm culture dishes	27100	10 Dishes/Pack	8.00 USD
ABCG2/BCRP1 Antibody, Clone 5D3	Mouse monocolonal antibody to human ABCG2/BCRP1	01428	100 µg	131.00 USD
ABCG2/BCRP1 Antibody, Clone 5D3, FITC-Conjugated	Mouse monoclonal antibody to human ABCG2/BCRP1 - FITC conjugated	10400	50 Tests	137.00 USD
Agar Leukocyte Conditioned Medium	Colony stimulating factors for assays of human hematopoietic progenitors	02300	25 mL	374.00 USD
ALDECOUNT® IVD Assay Kit with Lysis Buffer	To enumerate ALDH-bright cells in fresh and frozen clinical samples. for general diagnostic purposes. (Available in USA only)	01720	1 Kit	505.00 USD
ALDECOUNT® RUO Assay Kit with Lysis Buffer	To enumerate ALDH-bright cells in fresh and frozen clinical samples. for general diagnostic purposes. (RUO only, available worldwide)	01721	1 Kit	Request pricing
ALDEFLUOR™ Assay Buffer	Additional assay buffer for use with the ALDEFLUOR™ kit	01701	25 mL	44.00 USD