Embryonic stem cells & iPS cells (Human)

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Maintenance of embryonic stem cells and induced pluripotent stem cells

Maintain Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells

Maintenance of hiPSCs and hESCs requires careful technique and rigorously quality-controlled culture environments to ensure retention of pluripotency, stability and survival of these cells. mTeSR™1 and TeSR™2 are standardized and scalable systems for the culture and expansion of hiPSCs and hESCs feeder-free for research, screening, banking and clinical applications.

Reprogramming to induced pluripotent stem cells

Cellular Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells

Cellular reprogramming involves the transformation of somatic cells into induced pluripotent stem cells (iPSC) by the forced expression of defined factors under appropriate culture conditions. Advances in the iPSC field surround the development of safe, simple and efficient methods for reprogramming coupled with defined and standardized culture conditions.

Differentiation of embryonic stem cells and induced pluripotent stem cells

Differentiate Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells

Many protocols to differentiate hiPSCs and hESCs require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions. AggreWell™ plates allow users to control the size and shape of the EBs allowing for reproducibility, optimization of differentiation protocols to specific lineages, standardization of the production of EBs and reduction of variability associated with hiPSC and hESC differentiation.

Cryopreservation of embryonic stem cells and induced pluripotent stem cells

Cryopreserve Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells

Cryopreservation is a critical step in the storage and transportation of hiPSCs and hESCs. Efficient cryopreservation methods must offer high thawing efficiencies and maintain the pluripotency and differentiation potential of the cells.

Characterization of embryonic stem cells and induced pluripotent stem cells

Characterize Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells

Undifferentiated hiPSCs and hESCs can be characterized by the expression of cell surface antigens and intracellular transcription factors. A complete set of primary antibodies is available to enable the characterization of undifferentiated hiPSCs and hESCs.

Cell isolation of embryonic stem cells and induced pluripotent stem cells

Isolate Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells

Generation of clinically-useful cell types derived from hiPSC and hESC will rely on the optimization of relevant differentiation protocols. Due to issues with teratoma formation by undifferentiated cells and potential for non-specific differentiation, there is a need for methods to purify desired cell types of interest from a mixed cell population. The EasySep^® magnetic based cell isolation system allows for separation of these cell types.

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Product Name	Description	Catalog #	Size	Price
37 µm Reversible Strainer, large	37 µm Reversible Strainer, large	27250	12 per box	59.00 USD
37 µm Reversible Strainer, small	37 µm Reversible Strainer, small	27215	20 per box	53.00 USD
6-Well Ultra-Low Adherent Plates For Suspension Cultures	6-well ultra-low adherent plates for suspension cultures	27145	6 Plates/Pack	148.00 USD
ACCUMAX™	ACCUMAX™ cell detachment solution	07921	100 mL	93.00 USD
AggreWell™ Medium	Defined, serum-free medium for generation and culture of embryoid bodies using AggreWell™ plates	05893	100 mL	137.00 USD
AggreWell™ Rinsing Solution	AggreWell™ Rinsing Solution	07010	100 mL	35.00 USD
AggreWell™400	8 wells, each contains approximately 1,200 microwells with a diameter of 400 µm	27845	1 Plate	94.00 USD
AggreWell™400	8 wells, each contains approximately 1,200 microwells with a diameter of 400 µm	27945	5 Plates	443.00 USD
AggreWell™400Ex	6 wells, each contains approximately 4,700 microwells with a diameter of 400 µm	27940	5 plates	443.00 USD
AggreWell™400Ex	6 wells, each contains approximately 4,700 microwells with a diameter of 400 µm	27840	1 plate	94.00 USD